Screening Suitable Reference Genes for qRT-PCR in Different Strains of Phaeocystis globosa
Expression stability of seven candidate reference genes in different growth stages of three Pha￣eocystis globosa strains with different colony-formation ability was studied.Results showed that the optimal reference genes in colony-forming strain PG-1 are Ubiquitin,β-tubulin and 18S rRNA.α-tubulin,18S rRNA,β-tubulin and EF-1α are the best combination for another colony-forming strain PG-2.Therefore,β-tubulin and 18S rRNA were stably expressed in the colony-forming strains.The most stable reference genes in non-colony-forming strain PG-3 were Ubiquitin and α-tubulin.EF-1α,Ubiquitin and 18S rRNA could be used for the standardized analysis of gene expression level among three P.globosa strains.This study provides a basis for a more accurate study on the gene expression levels of P.globosa in different strains and different growth stages,especially verifying the key genes of colony formation.
万方数据
维普
