首页|不同氮效率小麦品种苗期根系氮代谢及其吸收能力差异分析

不同氮效率小麦品种苗期根系氮代谢及其吸收能力差异分析

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为了解不同氮效率小麦品种根系氮代谢特征及其吸收能力的差异,明确小麦氮高效利用的生理机制,在水培条件下,研究了氮高效小麦品种漯麦18和氮低效小麦品种西农509的根系氮代谢特征和对NO3-、NH4+吸收的动力学特征.结果表明,漯麦18的根系GS活性、硝酸还原酶活性、游离氨基酸含量、可溶性蛋白质含量均高于西农509;而西农509的根系硝态氮和铵态氮含量高于漯麦18;漯麦18根系对NO3-、NH+吸收的最大吸收速率(Vmax)显著高于西农509;漯麦18根系对NO3-、NH4+的亲和力(以Km的倒数衡量)低于西农509.结果说明,氮高效型小麦品种根系对NO3-、NH4+的吸收能力和同化能力均显著高于氮低效型小麦品种;小麦根系对NO3-、NH4+的吸收和同化是相互促进的关系.
Analysis of Nitrogen Metabolism in Roots and Uptake Characteristic of Wheat Cultivars with Different Nitrogen Efficiency at Seedling Stage
In order to clarify the physiological mechanisms of higher nitrogen use efficiency of wheat,the difference of nitrogen metabolism in roots and uptake characteristics of wheat cultivars with different nitrogen efficiency were investigated.Under hydroponic conditions,the nitrogen metabolism in roots and uptake kinetics to NO3-and NH4+ of N high efficiency wheat variety Luomai 18 and N low efficiency wheat variety Xinong 509 were studied.The results showed that the GS activity,NR activity,free amino acids concentration and soluble protein concentration in root of Luomai 18 were higher than those of Xinong 509.But the nitrate concentration and ammonium concentration in root of Xinong 509 were higher than those of Luomai 18.The maximum absorption rate(Vmax) to NO3-and NH4+ of Luomai 18 were higher than those of Xinong 509.But the affinity(1/Km) of Xinong 509 was higher than that of Luomai 18.In conclusion,the root absorptive capacity to NO3-and NH4+ and assimilative capacity of Luomai 18 was significantly higher than that of Xinong 509.There is a mutually reinforcing relationship between wheat roots absorption and assimilation to NO3-and NH4+.

WheatRootNitrogen metabolismUptake kinetics

熊淑萍、吴克远、王小纯、吴懿鑫、杜盼、马新明

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河南农业大学农学院/河南粮食作物协调创新中心/小麦玉米作物学国家重点实验室,河南郑州450002

河南农业大学生命科学学院,河南郑州450002

小麦 根系 氮代谢 吸收动力学特征

国家自然科学基金河南省现代农业(小麦)产业技术体系技术创新团队项目

31301281S2010-01-G04

2016

麦类作物学报
西北农林科技大学 国家小麦工程技术研究中心

麦类作物学报

CSTPCDCSCD北大核心
影响因子:0.886
ISSN:1009-1041
年,卷(期):2016.36(3)
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