Identification of Translocation Line of Triticum aestivum-Lolium perenne L.and Analysis on Grain Filling and Yield Traits of Its Offsprings
In order to explore the effect of exogenous chromosome or fragment on grain filling and yield of wheat,the offsprings of "Ningchun 4 × Lolium perenne" distant hybridization were used as materials in this study.The chromosome composition of BC1 hybrid offsprings was identified based on genomic in situ hybridization(GISH) using Lolium perenne L.DNA as probe and molecular markers,and characteristics of grain filling and yield traits in BC1F3 offsprings were analyzed.The results showed that,No.ND99 had two GISH signals of small fragment translocation types in top,and was true hybrid;The BC1F3 offsprings of ND99 were divided into awned type(ND99A) and awnless type (ND99B),and a molecular marker 3EST-185 could identify the two types.The changes of grain volume,fresh weight,dry weight,and water at filling stage in variety Ningchun 4 and translocation offsprings(ND99A,ND99B) showed a trend of first increasing and then decreasing;The filling rate of Ningchun 4 and ND99A showed a double-peak curve,but ND99B showed many peaks.The filling stage of Ningchun 4 was early in time,with long rapid growth period and biggest plumpness index of grain and kernel weight per spike.The filling time of ND99A showed later,with short extending period and lowest plumpness index,and kernel weight per spike was lower than that of Ningchun 4.The filling peak of ND99B appeared earlier and bigger,with short extending period and lowest kernel weight per spike.In the yield traits,panicle length,kernel number and fertile spikelet number per spike of translocation lines were apparently higher than those of Ningchun 4,which could be one of the key factors affecting grain filling.
NETL
NSTL
万方数据
维普
