Cloning and Yeast Expression of 3-ketoacyl-coenzyme Synthase (KCS) Gene in Wheat(Triticum aestivum L.)
Very-long-chain fatty acids (VLCFAs) are the synthetic substrates for many important substances in the living body.The KCS gene encodes 3-ketoacyl CoA synthase,a rate-limiting enzyme in the synthesis of VLCFAs,catalyzing the condensation reaction of VLCFAs elongation process.In order to identify the function of KCS gene in biosynthesis of VLCFAs,the homologous cloning strategy was used to clone KCS gene from wheat(Triticum aestivum L.),the coding sequence was analysed by a series of bioinformatics software and expressed in yeast (Saccharomyces cerevisiae).The result showed that the open reading frame of TaKCS6gene is 1 287 bp,which encodes 428amino acid residues.Functional domain analysis predicted that TaKCS6 protein belonging to KCSs protein family,contains FAE1_CUT1_RppA and ACP_syn_Ⅲ_C domains.The amino acid sequences of TaKCS6,AtKCS6 and KCS6 from other plants are conservative in two functional domains and activity sites.Yeast expression of TaKCS6 showed that encoded protein is involved in the elongation of VLCFAs more than 24 carbons.
NETL
NSTL
万方数据
维普
