Developmental Characteristics of Starch Granule in Endosperm of Tibetan Winter Hulless Barley
To study the formation and growth of hulless barley endosperm starch and starch granules,hulless barley Dongqing 8(cultivated in Tibet) was chosen as the target material to analyze endosperm filling rate,starch accumulation as well as the formation,2D area and size of starch granules in the process of granule development.The results showed that during the development of endosperm,dry matter auumulation and starch accumulation perform an S curve,and are positively related to each other.On the 5 days post anthesis(DPA),A-type starch granules with small sizes and 2D areas can be sparsely seen in hulless barley endosperm.During 10 to 15 DPA,the number of small A-type starch granules increased significantly,but their size and 2D area showed a slow increase.On 20 DPA,B-type small starch granules may come into being and they will be combined with A-type small starch granules to form a composite polymer.On 25 DPA,the size of A-type starch granules was above 10 μm,and the size of B-type starch granules ranged from 5 to 10μm,and the size of C-type starch granules was less than 5 μm.During 30 to 55 DPA,the number and 2D areas of starch granules with different sizes increased a lot,and the number of C-type starch granules grew until the granules mature.Diameter of starch granules in mature granule endosperm of hulless barley ranged from 1.45 μm to 44.76 μm,and the 2D area ranged from 3.56μm2 to 1 750.8μm2.A-type starch granules is roughly spherical with smooth surface.C-type starch granules adhered to surface of some A-type starch granules.Btype starch granules are shaped in sub-spherical and oval.Shape of type-C starch granules had greater variation than that of B-type starch granules.
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维普
