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合成六倍体小麦品系籽粒相关性状的QTL定位

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合成六倍体小麦作为一种重要的遗传资源,在籽粒相关性状的研究中起着关键作用.为进一步挖掘合成六倍体小麦籽粒相关性状的数量性状位点(quantitative trait loci,QTL),本研究以西农389与合成六倍体小麦KU2098杂交得到的154个重组自交系(recombinant inbred lines,RIL)群体为材料,用小麦55K SNP芯片构建遗传图谱,对粒长、粒宽、粒面积、粒周长、粒长宽比、千粒重、蛋白质含量、淀粉含量、湿面筋含量共9个籽粒相关性状进行了 QTL定位分析.结果表明,在除7B染色体外的20条染色体上共鉴定出84个QTL;这些QTL的LOD值在2.54~39.9之间,解释了 0.91%~43.41%表型变异;84个QTL包括31个主要 QTL 和 10 个稳定 QTL.在 1A(1)、1B(1)、1D(1)、2D(1)、4D(1)、5A(1)、5D(1)、6A(2)、6B(2)和 7D(1)染色体上共鉴定出12个与籽粒性状相关的QTL簇.在QTL簇C8对应的遗传区间中,筛选到2个有关籽粒性状的基因;在QTL簇C3的遗传区间中,筛选到4个有关品质性状的基因.鉴定出的QTL可为小麦产量和品质改良提供参考.
QTL Mapping for Kernel-Related Traits from Synthetic Hexaploid Wheat Lines
As a crucial genetic resource,synthetic hexaploid wheat plays a key role in studying of ker-nel-related traits.In order to further explore the quantitative trait loci(QTL)of kernel-related traits in wheat,a RIL population consisting of 154 lines derived from a cross between XN389 and a synthetic hexaploid wheat(KU2098)was used as materials.Wheat 55K SNP array was used to construct the genetic map and QTLs for nine kernel-related traits were identified,including kernel length(KL),kernel width(KW),kernel area(KA),kernel perimeter(KP),kernel length width ratio(KLWR),thousand-kernel weight(TKW),kernel protein content(KPC),SC(starch content)and wet gluten content(WGC).A total of 84 additive QTLs were identified across 21 wheat chromosomes,except for chromosome 7B.The individual QTL explained the phenotypic variation from 0.91%to 43.41%,with LOD values ranging from 2.54 to 39.96.Among them,31 major and 10 stable QTLs were iden-tified.A total of twelve QTL clusters,encompassing QTLs associated with kernel-related traits,were identified on chromosomes 1A(1),1B(1),1D(1),2D(1),4D(1),5A(1),5D(1),6A(2),6B(2)and 7D(1).In the corresponding physical interval of QTL cluster C8,two candidate genes re-lated to kernel traits were identified.Four candidate genes related to quality traits were identified in physical region of QTL cluster C3.The QTLs can provide reference for yield and quality improvement of wheat.

Synthetic hexaploid wheatWheat 55K SNPKernel traitsQTLs

孙迎凯、李翔、刘天相、张冰月、赵艺清、关露露、贾亚涛、马超、沈仲、赵力克、胡银岗、吉万全、史学芬、白海波、王中华

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西北农林科技大学农学院作物抗逆与高效生产全国重点实验室,陕西杨陵 712100

鄂尔多斯农牧科学院,内蒙古鄂尔多斯 017000

宁夏农林科学院农业生物技术研究中心,宁夏银川 750002

合成六倍体小麦 小麦55K SNP 籽粒性状 QTLs

2025

麦类作物学报
西北农林科技大学 国家小麦工程技术研究中心

麦类作物学报

北大核心
影响因子:0.886
ISSN:1009-1041
年,卷(期):2025.45(1)