Evaluation of Molecular Markers for Wheat Stripe Rust Resistance Genes Yr5,Yr9 and Yr 18
The traits of Yr5,Yr9(1B/1R),and Yr18 genes play an important role in wheat breeding in China,and the molecular markers STS9/10 of Yr5,AF1/4,D15,20H of Yr9(1B/1R)and csLV34,cssfr1～cssfr5 of Yr18 were effectively tested by applying near isogenic lines under background of Av-ocet S and lines with known Yr genes.The results showed that the specific molecular markers of Yr5 gene STS9/10,Yr9(1B/1R)gene AF1/4,D15 and 20H could accurately identify the corresponding resistance genes in different genetic background materials.Yr18 gene molecular marker cssfr2 could accurately detect the materials not-carrying Yr18,and detect allelic variation of Yr18 in Avocet S*6/Yr5,Avocet S*6/Yr24,and Avocet S*6/Yr27.There fore,the linkage marker STS9/10 of Yr5 gene,the molecular markers AF1/4,D15 and 20H of Yr9(1B/1R)gene can effectively detect the tar-get gene,and the combination of several molecular markers of Yr18 gene not only effectively detected the target gene but also identified the allelic variation of this site.
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