摘要
Yr5、Yr9(1B/1R)、Yrr18基因的 自身性状或连锁性状在中国小麦育种中具有重要的应用前景.本研究运用以Avocet S为背景的单基因近等基因系及其对应基因的载体品种,对Yr5基因的连锁标记STS9/10,Yr9(1B/1R)基因的分子标记 AF1/4、D15、20H,Yr18基因的分子标记csLV34、cssfr1～cssfr5进行有效性验证.结果表明,Yr5基因的特异性分子标记STS9/10、Yr9(1B/1R)基因的特异性分子标记AF1/4、D15和20H均能够准确识别不同遗传背景材料中的相应抗性基因;Yr18基因分子标记cssfr2能够准确检测Yr18的非载体材料,并检测出Avocet S*6/Yr5、Avocet S*6/Yr24、Avocet S*6/Yr27三个材料具有Yr 18的等位变异.这说明Yr5基因的连锁标记STS9/10,Yr9(1B/1R)基因的分子标记AF1/4、D15、20H能对目的基因进行有效检测,Yr18基因的几个分子标记结合使用不仅能够对目的基因进行有效检测,而且能够识别该位点的等位变异.
Abstract
The traits of Yr5,Yr9(1B/1R),and Yr18 genes play an important role in wheat breeding in China,and the molecular markers STS9/10 of Yr5,AF1/4,D15,20H of Yr9(1B/1R)and csLV34,cssfr1～cssfr5 of Yr18 were effectively tested by applying near isogenic lines under background of Av-ocet S and lines with known Yr genes.The results showed that the specific molecular markers of Yr5 gene STS9/10,Yr9(1B/1R)gene AF1/4,D15 and 20H could accurately identify the corresponding resistance genes in different genetic background materials.Yr18 gene molecular marker cssfr2 could accurately detect the materials not-carrying Yr18,and detect allelic variation of Yr18 in Avocet S*6/Yr5,Avocet S*6/Yr24,and Avocet S*6/Yr27.There fore,the linkage marker STS9/10 of Yr5 gene,the molecular markers AF1/4,D15 and 20H of Yr9(1B/1R)gene can effectively detect the tar-get gene,and the combination of several molecular markers of Yr18 gene not only effectively detected the target gene but also identified the allelic variation of this site.
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