Effect of Bestatin and MeJA on the Low Temperature Tolerance in Hulless Barley Seedlings
To investigate the regulatory effect of the jasmonic acid(JA)signaling pathway on low-tem-perature tolerance in hulless barley seedlings,this study utilized 'Kunlun 15' as the experimental ma-terial.The agronomic traits and physiological indicators were measured under low-temperature stress treated with Bestatin and methyl jasmonate(MeJA),and the expression levels of JA synthesis and regulation-associated genes in leaves and roots were examined.The result indicated that low-tempera-ture stress substantially decreased plant height,root length,plant dry(fresh)weight,root dry(fresh)weight,and SPAD value in hulless barley,indicating a marked inhibitory effect on growth.Treat-ment with either Bestatin or MeJA under low-temperature stress notably enhanced the accumulation of soluble sugars and free proline in both leaves and roots,increased the activity of antioxidant en-zymes(SOD,POD,CAT),and significantly induced the gene expression of JA synthesis and signa-ling pathways as well as low temperature tolerance-related genes regulated by JA in hulless barley.These responses suggest that Bestatin and MeJA treatments mitigate low-temperature stress damage in hulless barley by improving its low-temperature resistance.The study elucidated consistent regula-tory patterns in agronomic traits,physiological indicators,and gene expression induced by Bestatin and MeJA treatments in hulless barley.
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