首页|等离子磁性微球的制备及其蛋白检测分析

等离子磁性微球的制备及其蛋白检测分析

扫码查看
液态芯片技术以其多元同时检测的优势在生物分析领域得到了广泛的应用.然而,相比其他接近单分子检测灵敏度的生物分析检测技术,液态芯片技术的灵敏度还有待进一步提高.本研究以等离子磁性微球取代液态芯片技术中传统的微球,构建了具有荧光增强特性的新型液态芯片检测平台.研究表明,本文制备的等离子磁性荧光微球可以实现对特定荧光分子达到 70 倍的荧光增强效应,构建的新型液态芯片检测平台对蛋白类肿瘤标志物的检测灵敏度也可以提高 2 个数量级,为传统液态芯片技术检测灵敏度的提高提供了方法学借鉴.
Synthesis and Application of Plasmonic Magnetic Microbeads Featured with Signal Amplification Property
As a signal amplification method,plasmon-enhanced fluorescence technology demonstrated significant potential in practical application in the field of environmental-and biological-assay.Therefore,it is imperative to develop a method for the scalable and reproducible synthesis plasmon-enhanced fluorescence carrier,which plays a vital role in the commercialization of the technology.In this study,plasmonic magnetic microbeads were prepared by magnetic loading and polydopamine coating and subsequent noble metal nano island functionalization using polymer microbeads as the template.It is found that the prepared microbeads can be rapidly separated from the solution under an external magnet,and more 70-fold fluorescence enhancement can be obtained for dye CF647.By comparing with polystyrene bead-based suspension microarray,100-fold detection sensitivity can be achieved for biomarker CEA protein.

plasmon-enhanced fluorescenceCEAsuspension microarraymicrobead

曹本妹、李鑫、余龙、袁超

展开 >

广东石油化工学院 环境科学与工程学院,广东 茂名 525000

等离子荧光增强 微球 肿瘤标志物 液态芯片

广东石油化工学院人才引进项目茂名市科技计划项目

2022rcyj20022023385

2024

广东石油化工学院学报
广东石油化工学院

广东石油化工学院学报

影响因子:0.2
ISSN:2095-2562
年,卷(期):2024.34(1)
  • 10