Construction and Evaluation of Yeast cDNA Library of Guava Fruit
Yeast library is an important basis for studying protein interaction and transcriptional regulation.Constructing yeast cDNA library of guava fruit is an important way to study the complex molecular mechanism of postharvest maturation of guava fruit.Firstly,high quality mRNA of guava was obtained,then double-stranded cDNA library was synthesized,and the cDNA library was constructed into yeast pGADT7-Dest expression vector by Gateway site-specific recombinant method.The results showed that the capacity of the secondary yeast cDNA library was about 1.56×107 cfu,the length of inserted cDNA fragments was 0.85～5.00 kb,the average length was greater than 1.00 kb,and the polymorphism was abundant,indicating that the quality of the library was advanced.The construction of yeast cDNA library laid the experimental foundation for harvesting and fruit maturation key transcription factors and systematically studying the transcriptional regulation mechanism of fruit functional genes.
万方数据
维普
