Colorimetric Bioensor Based on NrGO-Hemin-Au NPs Nanozyme for Detecting Low-density Lipoprotein
In this work,a novel nanozyme named nitrogen-doped reduced graphene oxide-hemin-gold nanoparticles(NrGO-Hemin-Au NPs)with excellent peroxidase-like property was designed by self-agitation method.And LDL aptamer(LDLApt)was modified on the nanozyme surface to form a recognition probe(NrGO-Hemin-Au NPs-LDLApt).In the presence of LDL,the capture probe(LDLApt)and the recognition probe can specifically recognize LDL,thus forming the sandwich structure composites of NrGO-Hemin-Au NPs-LDLApt-LDL-LDLApt.The composites can catalyze oxidizing the colorless 3',3',5,5'-tetramethylbenzidine(TMB)to blue color oxidation state TMB(ox TMB),resulting in the absorption intensity changing at 652 nm of ox TMB.Under optimal experimental conditions,the absorption intensity had a good liner relationship with the LDL in the range of 100.0～1100.0 ng/mL,and the detection limit reached 28.5 ng/mL.The LDL colorimetric sensor was successfully applied to human serum samples,providing a nice platform for detecting cardiovascular disease biomarker.
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