Expression of UbcH7 and 53BP1 gene in colorectal cancer and its clinical value
Objective To investigate the expression and clinical significance of ubiquitin crosslinking enzyme L3(UbcH7)and p53-binding protein 1(53BP1)in colorectal cancer.Methods Ninety eight patients with colorectal cancer were selected as the study objects.Real-time fluorescent quantitative PCR(qRT-PCR)was used to detect UbcH7 and 53BP1 genes in colorectal cancer tumor tissues and adjacent normal tissues mRNA expression.The ex-pression of UbcH7 and 53BP1 gene protein was detected by immunohistochemical method(SP method).The mRNA and protein expression correlation of UbcH7 and 53BP1 genes in colorectal tumor tissues were analyzed by Pearson and Spearman methods.ROC curve was drawn to analyze the predictive efficacy of UbcH7 and 53BP1 mRNA ex-pression in colorectal tumors in 3-year survival.Kaplan-Meier method was used to analyze the relationship between the expression of UbcH7 and 53BP1 gene protein in colorectal tumor tissue and the 3-year survival rate.Results The mRNA expression and protein positive rate of UbcH7 gene in colorectal tumor tissue were significantly higher than those in adjacent normal tissues,and the mRNA expression and protein positive rate of 53BP1 gene were signifi-cantly lower than those in adjacent normal tissues(all P＜0.05).The mRNA expressions of UbcH7 and 53BP1 genes in colorectal tumor tissues were negatively correlated(r=-0.626,P＜0.05).The expression of UbcH7 and 53BP1 gene protein in colorectal tumor tissue was negatively correlated(r=-0.795,P＜0.05).The positive rate of UbcH7 protein expression and the negative rate of 53BP1 protein expression in colorectal cancer patients with stage Ⅲ+Ⅳ and N1+N2 lymph node metastasis was significantly higher than those in colorectal cancer patients with stage Ⅰ+Ⅱ and N0 lymph node metastasis(all P＜0.05).The cut-off value,sensitivity,specificity and the area under the curve(AUC)of UbcH7 gene mRNA in colorectal tumor tissue to predict 3-year survival in colorectal cancer patients were 1.17,0.789,0.824 and 0.726,respectively.The cut-off value,sensitivity,specificity and AUC of 53BP1 gene mRNA in colorectal tumor tissue to predict 3-year survival in colorectal cancer patients was 0.73,0.815,0.791 and 0.804,respectively.The 3-year survival rate of patients with positive UbcH7 expression was lower than that of patients with negative UBCH7 expression(P＜0.05).The 3-year survival rate of patients with positive expression of 53BP1 was higher than that of patients with negative expression(P＜0.05).Conclusions The expression level of UbcH7 in colorectal tumor tissue is increased,and the expression level of 53BP1 is decreased,which is closely related to TNM stage,lymph node meta-stasis and 3-year survival rate,and can be used as a marker for evaluating the condition and prognosis of colorectal can-cer patients.
Colorectal cancerUbiquitin cross-linking enzyme L3P53-binding protein 1Clinical value
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