Effect of S1PR3 on apoptosis of renal tubular eoithelial cells treated with lipopolvsaccharide
Objective To investigate the effect of sphingosine 1-phosphate receptor 3(S1PR3)on apoptosis of re-nal tubular epithelial cells treated with lipopolysaccharide(LPS).Methods The cultured rat renal tubular cells(NRK-52E)were divided into control group,LPS group and S1PR3 agonist+LPS group.The control group was not treated;LPS group was stimulated by LPS(20μg/ml)for 12 h and centrifuged.S1PR3 agonist+LPS group was pret-reated with S1PR3 agonist KRX-725 for 2 h and then stimulated with LPS(20 μg/ml)for 12 h.Flow cytometry was used to detect the differences in cell apoptosis rate,calcium ion expression and caspase-3 expression among all groups.ELISA kit was used to detect the differences in the expression of Calpain 1 and Calpain 2.Western Blot was used to detect the differences in the expression of S1PR3 among all groups.Results The NRK-52E cell model of sepsis was successfully established after LPS treatment.Apoptosis rate,S1 PR3 expression,calcium ion concentration,Calpain 1,Calpain 2 expression and caspase-3 expression were significantly increased in LPS group and S1PR3 agonist+LPS group(allP＜0.05).Compared with LPS group,apoptosis rate,calcium ion concentration,Calpain 1,Calpain 2 ex-pression and caspase-3 expression in S1PR3 agonist+LPS group were significantly increased(all P＜0.05),and S1PR3 expression was significantly increased(P＜0.05).Conclusions The activation of S1PR3 can increase the concentration of calcium ions in renal tubular epithelial cells,and thus activate the apoptosis signaling pathway of cas-pase-3 cells,leading to the increase of apoptosis of renal tubular epithelial cells in sepsis.
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