Exploration on the impact of fever on polarization of macrophage infected with Salmonella based on RNA-sequencing
Objective To investigate the impact of temperature on polarization of macrophage infected with Sal-monella based on the high-through RNA-sequencing technique.Methods The model of macrophage infected with Salmonella was established under different temperature conditions including 37℃,38℃,39℃ and 40℃.RNA se-quencing was used to analyze the common differentially expressed genes between the Salmonella typhi infected and non Salmonella typhi infected groups under various temperature conditions.Transcription analysis and trend analysis were performed on the polarization related genes of macrophages M1 and M2.Results One thousand two hundred and fifty six up-regulated and 1231 down-regulated co-expression genes were obtained by RNA-sequencing,and the ge-nes related to M1 or M2 polarization of macrophage were analyzed by transcription level and trend analysis;then,it was found that the increase of temperature to a degree less than 3 9 ℃ promoted the macrophage polarization to M1.However,M1 polarization of macrophage attenuates from 39 ℃ to 40 ℃.Meanwhile,the increased temperature enhanced the pol-arization of macrophage to M2 state.Conclusions Proper fever favors the polarization of macrophage to M1 state,while extreme temperature induces macrophage polarization earlier to M2 state that is prone to immune evasion.
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