Effects of microRNA-26a-5p on proliferation,migration and bubbling of vascular smooth muscle cells treated with oxidized low-density lipoprotein
Objective To investigate the effect of microRNA-26a-5p on vascular smooth muscle cells(VSMCs)proliferation,migration and foaminess during atherosclerosis(AS).Methods VSMCs were treated with oxidized low-density lipoprotein(ox-LDL)to construct a VSMCs model during AS simulation.The miR-26a-5p mimic and its negative control mimic(NC mimic)were transfected into the cells,and the cells were divided into the control group,ox-LDL group,ox-LDL+NC mimic group,and ox-LDL+miR-26a-5p mimic group.Quantitative real time-poly-merase chain reaction(qRT-PCR)was used to detect the expression of miR-26a-5p.Cell Countingkit-8(CCK-8)and 5-ethynyl-2'-deoxyuridine(EDU)staining were used to assess cell viability.Transwell and wound healing assay was used to analyze cell migration,and oil red O staining(ORO)was performed to observe the lipid droplet accumulation in cells.Immunofluorescence(IF)assay was used to observe the α-SMA expression in cells.Results The miR-26a-5p level in VSMCs decreased with increasing ox-LDL treatment concentration and time.Compared with the control group,the proliferation,migration,and invasion ability of VSMCs cells in the ox-LDL group were significantly enhanced,and lipid droplet aggregation increased,α-SMA signal weakened and foam degree increased.The proliferation,mi-gration and foaminess were up-regulated by ox-LDL,while miR-26a-5p mimic effectively reversed these changes.Conclusions Over-expression of miR-26a-5p downregulates proliferation,migration and foaming of VSMCs,and miR-26a-5p may be a new target for AS therapy.
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维普
