Effects of Nesfatin-1 on colonic motility and CRF signaling pathway in IBS-D rats
Objective To explore the effects of Nesfatin-1 on colonic motility and corticotropin-releasing factor(CRF)signaling pathway in rats with irritable bowel syndrome with predominant diarrhea(IBS-D).Methods Male SD rats were divided into control group,IBD-D group,Nesfatin-11 group,Nesfatin-12 group and Nesfatin-13 group.Except control group,rats in the other groups were made into IBS-D models.The rats inall the Nesfatin-1 groups were injected with Nesfatin-1(0.5,5.0,50.0 μmol/L)into the left vagus nerve complex,while those in control group and IBS-D group were given with the same volume of 0.9％sodium chloride solution.The colon transport function and small intestine propulsion rate of each group of rats was compared.The level of vasoactive intestinal peptide(VIP)in brain and colon tissues was detected by ELISA.The expressions of interstitial cells of cajal(ICC)marker c-kit and capsaicin receptor 1(TRPV1)in colon tissues were detected by immunohistochemistry.The expression levels of CRF,CRF-R1 and CRF-R2 proteins were detected by Western blot.Results The efflux time of glass ball in IBS-D group was shorter than that in control group(P＜0.05).VIP level in brain and colon tissues,and expression of CRF-R2 protein in colon tissues were lower than those in control group(all P＜0.05).The small intestine propulsion rate,av-erage optical density of c-kit and TRPV1 in colon tissues,and expressions of CRF and CRF-R1 proteins were higher than those in control group(all P＜0.05).The efflux time of glass ball in all the Nesfatin-lgroups was longer than that in IBS-D group(all P＜0.05).VIP level in brain and colon tissues,and expression of CRF-R2 protein in colon tissues were higher than those in IBS-D group(all P＜0.05).The small intestine propulsion rate,average optical density of c-kit and TRPV1 in colon tissues,and expressions of CRF and CRF-R1 proteins were lower than those in control group(all P＜0.05).Conclusions Nesfatin-1 can improve intestinal propulsion in IBS-D rats,which may be related to increasing VIP level,inhibiting ICC level and the activity of CRF signaling pathway.
万方数据
维普
