Study on the relieving and protective effects of lycium barbarum polysaccharides on the liver of rats model with intrahepatic cholestasis model
Objective To study the relieving and protective effects of lycium barbarum polysaccharides(LBP)on liver injury in rats model with intrahepatic cholestasis.Methods The model of intrahepatic cholestasis in SPF male sprague-dawley(SD)rats was established,and the negative control group,positive control group,model group,LBP dose groups of 50,150 and 450 mg/kg were set up.The model group,each dose group of LBP,and positive control group was given 60 mg/kg α-Naphthalene isothiocyanate(ANIT)every other day,and from the second week,it was changed to every three days for a duration of six weeks.From the second week,different doses of LBP were given to each dose group,ursodeoxycholic acid was given to the positive control group,and distilled water was given to the model group.After six weeks,blood and liver tissue samples were collected to measure serum biochemical indicators,coagulation function indicators and white blood cell(WBC)classification count indicators.The content of inflamma-tory factors and the expression levels of TLR4,nuclear factor(NF)-κB,pNF-κB,and MyD88 proteins in rat liver tissue were measured,and the pathological changes in rat liver were observed.Results Compared to the negative control gro-up,the values of the serum γ-GGT,alkaline phosphatase(ALP),total bile acid(TBA),total bilirubin(TBiL),cholesterol(CHOL),ALT,AST levels,the blood WBC,lymphocyte(LYMP),neutrophile granulocyte(NEUT),mononuclear leuco-cyte(MONO),eosinophilic granulocyte(EO)counts,and fibrinogen(FIB)content increased in model group(all P＜0.05),and the concentration of TNF-α,IL-1β,IL-6 and macrophage inflammtory protein(MIP)-2 increased,and the levels of TLR4,pNF-κB and MyD88 proteins were upregulated(all P＜0.05).Pathological changes such as excessive inflamma-tion,bile duct hyperplasia,and fibrous tissue hyperplasia were observed,and the observation score increased in model gro-up(all P＜0.05).Compared with the model group,the LBP 450 mg/kg group serum transglutaminase(γ-GGT),TBA,TBiL,ALT,blood WBC,LYMP,NEUT counts,and FIB concentration all decreased(all P＜0.05),and the content of TNF-α,IL-1β,MIP-2 decreased,and the levels of TLR4,pNF-κB and MyD88 proteins were down regulated(all P＜0.05).The morphological scores decreased(all P＜0.05),and liver damage was alleviated.The level of TLR4 protein in the LBP 150 mg/kg group was down regulated(all P＜0.05).Conclusions LBP has alleviating and protective effects on liver damage in rats with intrahepatic cholestasis model and their mechanism may be related to downregulating TLR4/MyD88/NF-κB signaling pathway related proteins and inhibiting of excessive inflammatory response.
Lycium barbarum polysaccharidesCholestasisLiver protective effectTLR4/MyD88/NF-κB signal pathway
万方数据
维普
