Indolepropionic acid alleviates thioacetamide induced acute liver injury by promoting regulatory T cell levels
Objective To investigate the potential mechanisms of indolepropionic acid(IPA)in mitigating thioac-etamide(TAA)-induced acute liver injury,and ananlyze the role of regulatory T cells(Treg)in this process.Methods SPF-grade male C57BL/6 mice were randomly divided into a control group(injected with saline),a TAA group(TAA 100 mg/kg intraperitoneal injection),and an IPA group(TAA-induced model followed by IPA 20 mg/kg ga-vage),with ten mice in each group.Mouse serum and liver tissues were collected after 24 hours.alanine aminotrans-ferase(ALT),aspartate aminotransferase(AST),pro-inflammatory factors[interferon(IFN-γ),tumor necrosis factor(TNF-α),interleukin(IL)-1β,IL-6],and anti-inflammatory factors[transforming growth factor(TGF-β),IL-10]levels were measured by ELISA.Liver injury was assessed by histologically,and liver resident Treg levels were detected by immunofluorescence.In vitro,mice spleen was obtained and Naïve T cells(Naïve T)were selected to induce Treg.IPA was added during the culture process to explore its effect on Treg differentiation.ELISA was used to measure the secretion levels of anti-inflammatory factors TGF-β,IL-10 and the corresponding RNA expression levels.Results IPA significantly reduced TAA-induced acute liver injury.Liver function index ALT,AST,and pathological scores in the IPA group were significantly lower than those in the TAA group(all P＜0.05).Immunofluorescence revealed higher level of Treg in the liver of the IPA group(P＜0.05).ELISA results showed that pro-inflammatory factors IFN-γ,TNF-α,IL-1β,IL-6 in the liver of mice in the IPA group significantly decreased,while anti-inflammatory factors TGF-β,IL-10 levels significantly increased(all P＜0.05).In vitro experiments showed that IPA promoted Treg dif-ferentiation and expressed higher levels of Foxp3,as well as promoting the secretion and corresponding RNA ex-pression of anti-inflammatory factors TGF-β,IL-10.Conclusions IPA alleviated TAA induced acute liver injury by promoting the differentiation of Treg and the anti-inflammatory factor(TGF-β,IL-10)secretion,regulating liver im-mune balance and inhibiting liver inflammatory response.
Indolepropionic acidRegulatory T cellsThioacetamideLiver injury
万方数据
维普
