The correlation between PPARγ ligand-induced signal mediated pathway and VEGF-A,bFGF and HIF-1α in CCl4 hepatic fibrosis model rats by Qinghua Yudu formula
Objective Exploring the effect of Qinghua Yudu(QHYD)formula on the PPARγ ligand(PPARγ)in-duced signaling pathway in carbon tetrachloride induced liver fibrosis model rats and the relationship between related cytokines.Methods Fifty Sprague Dawley(SD)rats were randomly divided into the blank(normal)control group,model group,QHYD formula low-dose group,QHYD formula middle-dose group,QHYD formula high-dose group model(10 rats in each group).After successful modeling,the normal group was gavaged with an equal amount of sa-line.The low,medium,and high dose groups of QHYD formula were treated with 50,100,and 200 mg·kg-1·d-1 by gavage for eight weeks,respectively.The liver histopathology was observed.Fluorescence expression of PPARy and TLR2 was detected by immunofluorescence assay.The expressions of vascular endothelial growth factor-A(VEGF-A),basic fibroblast growth factor(bFGF),and hypoxia inducible factor-1α(HIF-1α)were analyzed by im-munohistochemical methods.Meanwhile,the expressions of cytokines intercellular adhesion molecule-1(ICAM-1),vascular endothelial cell adhesion molecule-1(VCAM-1)were detected by ELISA.Results Compared with the blank group,the expressions of toll-like receptor 2(TLR2),VEGF-A,bFGF,HIF-1α,ICAM-1,and VCAM-1 in each group was significantly increased.The PPARγ expression was reduced in the model group when compared to the blank group.After intervention with the QHYD formula,the PPARγ expression was enhanced.Conclusions QHYD formula can regulate PPARγ,VEGF-A and HIF-1α,and activate PPARγ and ligand-induced signal by regulating the production,migration,ad-hesion and contraction of angiogenic cytokines,so as to improve the liver injury of model rats,angiogenesis and vascular remodeling to achieve the role of anti-liver fibrosis.
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