Objective To investigate the effect of lncRNA HOTAIR on the proliferation and migration of oral squamous cell carcinoma cells and its potential molecular mechanism.Mehtods CAL27 cells were cultured in vitro and divided into control group,NC group(transfected with pcDNA plasmide),HOTAIR group(transfected with pcDNA-HOTAIR),HOTAIR+miR-122 group(transfected with pcDNA-HOTAIR and miR-122 mimic).RT-PCR was used to analyze the expression levels of HOTAIR and miR-122 in CAL27 cells.Cell count kit(CCK-8)was used to de-tect cell viability.Cell migration was detected by scratch test.Dual luciferase reporter gene assay was used to detect the regulatory effect of HOTAIR on miR-122 gene expression.Results Compared to the NC group,the expression of HO-TAIR was increased in the HOTAIR group(P＜0.05),while the expression of miR-122 was decreased(P＜0.05).Compared to the NC group,cell viability and migration were increased in HOTAIR group(all P＜0.05).Compared to the HOTAIR group,cell viability and migration were decreased in HOTAIR+miR-122 group(all P＜0.05).Dual luci-ferase reporter gene results showed that HOTAIR targets miR-122 and negatively regulated its expression.Conclusions HOTAIR can promote the proliferation and migration of CAL27 cells,and the mechanism may be related to the tar-geted inhibition of miR-122 gene expression.
维普
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