Objective To investigate the effect of circRNA_0008720 on the proliferation and invasion of breast cancer cells and its possible mechanism.Methods Real-time quantitative fluorescent PCR(qRT-PCR)was used to detect the expression of circRNA_0008720,miR-138-5p and SOX4 in breast cancer tissues,and the targeting relati-onship between them were determined by luciferase reporter gene assay.The EMT degree,proliferation and invasion ability of breast cancer cells were detected by CCK-8,Transwell and Western blot.Results circRNA_0008720 and SOX4 were up-regulated in breast cancer tissues and breast cancer cell lines,while miR-138-5p expression was de-creased.Knockdown the expression of circRNA_0008720 or up-regulate miR-138-5p could inhibit EMT process,proliferation and invasion activity of breast cancer cells;while inhibition of miR-138-5p or overexpression of SOX4 can partially reverse this effect.Conclusions circRNA_0008720 can up-regulate SOX4 expression through spon-ging miR-138-5p to promote EMT process,proliferation and invasion activity of breast cancer cells.
关键词
乳腺癌/环状RNA/微小RNA/上皮细胞-间充质转化
Key words
Breast cancer/circRNA/miRNA/Epithelial-mesenchymal transition
维普
万方数据