Objective To explore the mechanism of Berberine liquid in repairing bacterial infected skin wounds in mice by affecting the nuclear factor(NF)-κB signaling pathway.Methods Sixty nine-week-old male SPF C57BL/6J mice were selected to construct a methicillin-resistant staphylococcus aureus(MRSA)model.Fifty mice were ran-domly divided into model group,positive control group and Berberine liquid low(1.25％),medium(2.5％)and high dose groups(5％),with ten mice in each group.The healing rate and the number of colonies were calculated on the 3rd,7th and 14th day of administration,respectively.The pathological changes of wound tissue were observed after administration.The levels of interleukin-6(IL-6),tumor necrosis factor α(TNF-α),matrix metalloproteinase-2(MMP-2)and vascular endothelial growth factor(VEGF)were detected by enzyme-linked immunosorbent assay(ELISA).The mRNA expressions of toll-like receptor 2(TLR2),transforming growth factor-β-activated kinase-bin-ding protein 1(TAB 1)and NF-κB p65 were detected by RT-qPCR.The protein expressions of TLR2,TAB 1 and NF-κB p65 were detected by Western blot.Results With the duration of administration,the wound healing rate of mice in model groups gradually increased(P＜0.05),the number of clonies gradually decreased(P＜0.05).HE staining showed that the model group had significant granulation and more inflammatory cells.The low dose grop had a mild granulation and inflammatory cells;The medium dose group had no granulomatous tissue hyperplasia and slight in-flammatory cells.A few neutrophils were found in the high dose group.With the increase of administration,the ex-pression of IL-6,TNF-α and MMP-2 were decreased,and VEGF level was increased(all P＜0.05);the expressions of TLR2,TAB1,NF-κB p65 mRNA and protein were decreased(all P＜0.05).Conclusions Berberine liquid may attenuate the tis-sue inflammatory response of MRSA-infected skin wounds in mice by inhibiting the activation of the TLR2/NF-κB signa-ling pathway,and promote wound healing.
万方数据
维普
