Objective To investigate the effect of miR-122 targeting regulation of dual-specificity phosphatase 4(DUSP4)on proliferation of oral squamous cell carcinoma cells(OSCC).Methods OSCC(CAL27)cells were transfected with miR-122 mimic(miR-122 mimic group),miR-NC(miR-NC group),respectively,and a control group was set up.Cell viability was detected by MTT assay.The expression of DUSP4 protein was detected by Western blot.Dual luciferase activity assay was uesd to detect the effect of miR-122 on DUSP4 gene expression.MTT assay was uesd to detect the effect of DUSP4 on CAL27 cell viability.Results Seventy-two hours after transfected,the cell vi-ability of miR-122 mimic group was lower than that of NC group(P＜0.05),and there was no significant difference between NC group and control group(P＞0.05).The expression of DUSP4 protein in miR-122 mimic group was lower than that of NC group(P＜0.05),and there was no significant difference between NC group and control group(P＞0.05).The dual luciferase activity assay showd that miR-122 could inhibit the expression of DUSP4 genes(P＜0.05).MTT assay showed that transfection of DUSP4 overexpression plasmid could partially eliminate the inhibi-tory effect of miR-122 mimic on CAL27 cell viability,and improve the viability of CAL27 cells.Conclusions miR-122 inhibits the viability of CAL27 cells by targeted inhibition of DUSP4 expression.
维普
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