Relationship between high-risk HPV infection combined with cervical intraepithelial neoplasia and vaginal microenvironment and TAP1 and TAP2 gene polymorphisms
Relationship between high-risk HPV infection combined with cervical intraepithelial neoplasia and vaginal microenvironment and TAP1 and TAP2 gene polymorphisms
Objective To investigate the relationship between high-risk human papilloma virus(HR-HPV)infec-tion and cervial intraepithelial neoplasia(CIN)with vaginal microenvironment,TAP1,and TAP2 gene polymorph-isms.Methods A total of one-hundred and twenty patients with HR-HPV infection were selected,thinprep liqui-based cytology test(TCT)showed that sventy-five patients were complicated with CIN(HR-HPV infection combined with CIN group),and the other fourty-five patients were included in HR-HPV infection cervicitis group.Cervical and vaginal secretions were collected and sequenced by 16S rRNA technology.The distribution of vaginal microenviron-ment flora in the two groups was analyzed at the"phylum"and"genus"level by comparison with the Greengene dat-abase.TAP 1 and TAP2 gene polymorphisms were amplified by polymerase chain reaction(PCR)in peripheral venous blood,and the relationship between CIN,TAP1 and TAP2 gene polymorphisms in HR-HPV infection was analyzed.Results At the"phylum"level,the relative abundance of Firmicutes in the HR-HPV infection complicated CIN group was lower than that in the HR-HPV infection cervicitis group,Actinobacteria,Bacteroidetes,Proteobacteria,Tenericutes and Fusobacteria were higher than those in HR-HPV infected cervicitis group(all P＜0.05).At the"ge-nus"level,the Lactobacillus in the HR-HPV infected CIN group was lower than that in the HR-HPV infected cervic-itis group;Gardnerella,Prevotella,Atopobium,Ureaplasma,Sneathia,Streptococcus and Anaerococcus were higher than those in HR-HPV infected cervicitis group(all P＜0.05).There was no significant difference between the the-oretical and actual values of TAP1 and TAP2 polymorphic loci between the two groups(P＞0.05).The genotype dis-tribution of TAP 1 rs41549617 locus was statistically significant between the two groups(P＜0.05),and the AA geno-type and A allele frequencies in the HR-HPV infection combined with C1N group were higher than those in the HR-HPV infection cervicitis group(all P＜0.05).There was no significant difference in genotype distribution of TAP2 rs1 135216 between the two groups(P＞0.05).Conclusions HR-HPV infected patients can further develop CIN due to changes in vaginal microflora and TAP1 rs41549617 site.Detection of vaginal microflora and TAP1 rs41549617 site is helpful for risk stratification of HR-HPV infected patients.
关键词
高危型人乳头瘤病毒/宫颈上颈上皮内瘤变/阴道微环境/TAP1/TAP2
Key words
High-risk human papilloma virus/Cervical intraepithelial neoplasia/Vaginal microenvironment/TAP1/TAP2
维普
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