首页|辐照杀菌全蛋液贮藏期间抗氧化物活性及过敏源特性变化分析

辐照杀菌全蛋液贮藏期间抗氧化物活性及过敏源特性变化分析

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研究了对照组、巴氏杀菌组和辐照杀菌处理组(2,4,6 kGr)对4℃贮藏期间(0,10,20 d)全蛋液抗氧化物活性及卵白蛋白过敏源特性变化影响,抗氧化物活性指标包括羟自由基清除能力、DPPH自由基抑制率和还原力等指标.结果显示,辐照杀菌处理组(2,4,6 kGr)全蛋液在第10天时DPPH自由基和羟自由基抑制率显著高于对照处理组(p<0.05);第0天和第20天辐照杀菌2 kGr处理组巯基含量与对照组和巴氏杀菌处理组之间无显著差异(p>0.05);SDS-PAGE和Western-blot结果表明,贮藏期间辐照杀菌处理并没破坏全蛋液中重要过敏源——卵白蛋白.
The Changes of Antioxidation Parameters and Antigenicity Characteristics of Ovalbumin in Refrigerated-Storage Liquid Whole Egg Samples During Irradiation Sterilization Treatments
In order to investigate the changes of antioxidant parameters and ovalbumin antigenicity characteristics of ovalbumin in whole liquid egg samples of control,pasteurization and irradiation sterilization (2,4,6 kGr) treatments during refrigerated storage at 4 ℃ (0,10,20 days) for about 20 days,the antioxidant parameters include free sulfhydryl content,DPPH radical scavenging ability,hydroxyl radical scavenging ability and reducing powder.The samples of liquid whole egg under irradiation treatments (2,4,6 kGr) had significantly higher (p<0.05) DPPH and hydroxyl radical scavenging ability than the control group at 10 days.No significant difference (p>0.05) in free sulfhydryl contents is detected among whole liquid egg samples of control,pasteurization and irradiation 2 kGr ste rilization groups.The results of SDS-PAGE and Western-blot show that irradiation sterilization treatments could not destroy the main antigenicity (ovalbumin) in whole liquid egg samples.

irradiation sterilizationDPPH radical scavenging abilityhydroxyl radical scavenging abilityantigenicitywhole liquid egg

戴妍、韩兆鹏、范蓓、卢嘉、卢晓明

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北京德青源农业科技股份有限公司,北京102115

中国农业科学院原子能利用研究所,北京100193

辐照杀菌 DPPH自由基抑制率 羟自由基清除能力 过敏源 全蛋液

蛋制品加工技术研发与产业化示范公益性行业(农业)科研专项

2012BAD28B08201303084

2016

农产品加工·综合刊
山西省农机化研究院 山西省农产品加工装备技术管理站 山西省农业产业化协会

农产品加工·综合刊

影响因子:0.515
ISSN:1671-9646
年,卷(期):2016.(1)
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