Study on Separation and Purification of Functional Components in Chinese Yam Peel Extract
Using the recrystallization and column separation method for effect of Chinese yam peel extract components of allantoin and saponin sync separation.The results show that the selected AB-8 macroporous resin adsorption resin saponin,adsorption velocity is 2 mL/min;adsorption finished with 70% ethanol elution,eluting rate in more than 90%;washing liquid to collect and vacuum concentrating on its,then vacuum drying,light yellow saponin product,by legal institutions detection,its purity 71.5%,the yield is 0.17%;concentrate containing allantoin to crystallization,temperature is 0~4 ℃,time 24 h.The crystals with water as solvent,ratio of material to liquid is 1 ∶ 3 in case 2 times recrystallization,drying to obtain allantoin product purity of 85.8%,the yield is 0.25%.
Chinese yam peel extractfunctional componentsseparation and purification