Separation and Identification of Yeast in Beida Grape Wine
In order to obtain a dominant yeast strains at later stage of grape wine fermentation,isolation and purification of yeast in beida grape wine is performed and colony morphology is identified.Then PCR amplification of 26S rRNA of yeast isolates is conducted,further identification by gene sequence analysis.Results show that yeast colony number in beida grape wine is 3.4 × 107 CFU/mL,the yeast isolated strains Y3,Y5,Y13 are saccharomyces cerevisiae.Therefore,the advantage yeast in grape wine later fermentation is saccharomyces cerevisiae.