Aqueous Enzymatic Extraction of Black Soybean Oil and Analysis of Fatty Acid Composition
Aqueous enzymatic extraction of black soybean oil from black soybean assisted by ultrasound is studied.Effects of enzymatic hydrolysis conditions and ultrasonic pretreatment conditions on the extraction rate are explored,the optimum extraction condition is obtained as follows:ratio of stuff to water 1 ∶ 8 (g ∶ mL),dosage of alcalase 2.0％,pH 8.5,temperature 55 ℃,time 5 hours.Under this optimal conditions,the oil extraction efficiency reached 89.2％.The ultrasound irradiation at 420 W for 20 min followed by aqueous enzymatic extraction could increase the oil extraction efficiency to 94.5％,which is 5.3％ higher than that of non-irradiation with ultrasound.GC-MS is employed to analyze the components of fatty acid of black soybean oil.The main components of black soybean oil are ten kinds of fatty acid suchas palmitic acid 19.33％,linoleic acid 46.17％,oleic acid 18.73％,stearic acid 7.18％,total content of unsaturated fatty acids is 68.34％.
black soybean oilultrasonic-assisted extractionaqueous enzymatic extractionfatty acid composition
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