This work aims to investigate the differences in the content and antioxidant activity of phenolic compounds from various parts of mature Citrus aurantium L.fruits.Base on signal-factor experiment,the extraction yield of phenolic compounds is used as response value to establish a regression model with four factors.Phenolic compounds from various parts of fruit are extracted for content measurement.Their compositions are detected by HPLC-MS method,and then DPPH and ABTS free radical scavenging capacities and FRAP total antioxidant capacity are evaluated.The op timized parameters forextracting phenolic compounds from Citrus aurantium L.pulp are confirmed as follows:material to liquid is 1 ∶ 16 (g ∶ mL),ethanol concentration is 46％,pH value is 2.5,and extraction temperature is 39 ℃.Under such conditions,the extraction yield of phenolic compounds reached to 0.45％.The phenolic compounds of the fruit mainly existed as free,the contents of free polyphenols in various parts are ordered as pulp ＞ peel ＞ seed,and the contents of free flavonoids are ordered as peel ＞ seed ＞ pulp.The antioxidant abilities of different parts,including DPPH and ABTS free radical scavenging capacities and FRAP total antioxidant capacity,could all be ordered as pulp ＞ peel ＞ seed.
NETL
NSTL
维普
万方数据
