首页|蘑菇渣和稻秸堆肥中DOM与Cu2+的络合机制

蘑菇渣和稻秸堆肥中DOM与Cu2+的络合机制

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堆肥中溶解性有机质(DOM)对重金属具有显著的络合作用，影响其在环境中的迁移和转化。研究采用三维荧光-平行因子(EEM-PARAFAC)结合二维相关光谱(2DCOS)分析方法，系统解析蘑菇渣堆肥(MRC)及水稻秸秆堆肥(RSC)中DOM与Cu2+的络合机制。EEM-PARAFAC结果表明，MRC-DOM和RSC-DOM主要由类腐殖酸(C1)、类富里酸(C2)和类蛋白质(C3)组成，各组分分别占MRC-DOM总荧光强度的 43%、32%和 25%，占RSC-DOM的 39%、29%和 32%。2 种堆肥DOM与Cu2+作用过程中，3 类组分荧光强度均呈不断降低趋势，表明它们均与Cu2+发生了显著的络合作用。2 种堆肥中不同荧光组分(C1～C3)与Cu2+的有效猝灭常数(lg K)在 4。54～4。76之间，均表现为C3＞C1＞C2，表明类蛋白组分(C3)与Cu2+结合能力最强，其次为类腐殖酸(C1)，类富里酸(C2)最低。2DCOS结果显示，堆肥DOM中类蛋白荧光组分与Cu2+结合能力最强，而类富里酸优先与Cu2+发生反应。总体而言，MRC-DOM和RSC-DOM中类蛋白和类腐殖质组分是与Cu2+发生络合作用的关键物质，且 2 类堆肥DOM具有类似的Cu2+络合机制(包括活性位点、络合能力和反应顺序)。研究结果为进一步了解堆肥对土壤Cu2+迁移和转化行为的影响提供了重要的理论基础。
The Binding Properties of Cu2+onto Dissolved Organic Matter from Mushroom Residue and Rice Straw Compost
Dissolved organic matter(DOM)within compost readily complexes with heavy metals,influencing their migra-tion and transformation in the natural environment.In this study,three-dimensional fluorescence-parallel factor(EEM-PARAFAC)and two-dimensional correlation spectroscopy(2DCOS)protocol were used to comprehensively analyze the binding properties of Cu2+onto DOM derived from mushroom residue compost(MRC)and rice straw compost(RSC).The EEM-PARAFAC results show that the two compost-derived DOM primarily comprised humic-like acid(C1),fulvic-like acid(C2)and protein-like substances(C3).These components accounted for 43%,32%and 25%of the total fluores-cence for MRC-DOM,and 39%,29%and 32%for RSC-DOM,respectively.During the interaction between the compost-derived DOM and Cu2+,the fluorescence intensity of the three PARAFAC-derived components consistently decreased.The result indicate that both the MRC-DOM and RSC-DOM had significant complexing affinities with Cu2+.Moreover,the lg K of fluorescent components in MRC-and RSC-DOM was between 4.54 to 4.76,and followed the order of C3＞C1＞C2.The results suggest that the protein-like components exhibited a stronger binding ability to Cu2+than humic-like acid and fulvic-like acid substances in both types of compost DOM.2DCOS further confirm that protein-like fluorescent components had the highest binding affinity to Cu2+,while fulvic-like components exhibited a preferential reaction with Cu2+.In total,pro-tein-like and humic-like substances within MRC-and RSC-DOM emerged as pivotal components for complexing with Cu2+.Furthermore,both types of compost-derived DOM exhibited similar binding behaviors,including the active binding sites,capacity and preferential sequence.These findings provide an important theoretical basis for comprehending the impact of compost application on the migration and transformation behavior of soil-bound Cu2+.

compostdissolved organic matterCu2+excitation-emission matrix-parallel factor analysistwo-dimensional correlation spectroscopy

范行程、葛俊杰、谢越、任兰天、纪文超、范行军、王翔

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安徽科技学院资源与环境学院, 安徽 凤阳 233100

生物炭与农田土壤污染防治安徽省重点实验室, 安徽 蚌埠 233400

安徽科技学院农学院,安徽 凤阳 233100

堆肥 溶解性有机质 Cu2+ 三维荧光-平行因子分析 二维相关光谱

国家自然科学基金安徽省自然科学基金安徽省高校优秀青年科研项目安徽省高校优秀人才支持计划项目

521001142108085MD1402022AH030145gxyqZD2021126

2024

10.19741/j.issn.1673-4831.2023.0032

生态与农村环境学报
环境保护总局南京环境科学研究所

生态与农村环境学报

CSTPCDCHSSCD北大核心
影响因子：1.248
ISSN：1673-4831
年,卷(期)：2024.40(2)
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