奥奈达希瓦氏菌(Shewanella oneidensis)MR-1对氟西汀降解过程的转录组及功能基因分析
Transcriptomic and Functional Genes Analyses of Shewanella oneidensis MR-1 upon Degradation of Fluoxetine
万宇 1杭小帅 2王志刚 3张澜 2周莉 2尤晓慧 2朱冬冬 2王燕 2肖静 2陈翔2
作者信息
- 1. 扬州大学环境科学与工程学院,江苏 扬州 225009;生态环境部南京环境科学研究所,江苏 南京 210042
- 2. 生态环境部南京环境科学研究所,江苏 南京 210042
- 3. 扬州大学环境科学与工程学院,江苏 扬州 225009
- 折叠
摘要
该文研究了厌氧条件下奥奈达希瓦氏菌(Shewanella oneidensis)MR-1 对氟西汀的降解性能,并从转录组学分析遗传分子代谢机制.结果表明,Shewanella oneidensis MR-1 在厌氧条件下可有效降解体系中 93.12%的氟西汀,降解速率达 0.94 mg·L-1·h-1.采用Illumina高通量测序平台对降解氟西汀后的细菌与对照组细菌进行测序,通过基因本体数据库(GO)和京都基因与基因组百科全书数据库(KEGG)富集分析,结合筛选的高表达高上调的差异表达基因,得到了耐受和降解氟西汀的功能基因,其中包膜应激反应膜蛋白基因、ABC转运蛋白基因、噬菌体休克蛋白PspA基因、氧化应激防御蛋白基因等在Shewanella oneidensis MR-1 对环境的耐受中起重要作用;细胞色素C基因、硝基还原酶NfsB基因、乳酸利用蛋白基因等在氟西汀的降解转化中起关键作用.该研究从转录组水平分析了氟西汀的降解机制,可为Shewanella oneidensis MR-1 在环境修复中的应用提供参考依据.
Abstract
This paper studied the degradation performance of fluoxetine by Shewanella oneidensis MR-1 under anaerobic conditions and analyzed the genetic molecular metabolic mechanism from transcriptome.The removal rate of fluoxetine was 93.12%within 12 h,and the degradation rate was 0.94 mg·L-1·h-1.The transcriptome sequencing of Shewanella onei-densis MR-1 was performed using the Illumina high-throughput sequencing platform to understand the degradation mecha-nism of fluoxetine.Profiling and functional analysis of differentially expressed genes for tolerance and fluoxetine degradation using Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)revealed that envelope stress re-sponse membrane protein,ABC transporter,phage shock protein PspA and oxidative stress defense protein played important roles in the tolerance of Shewanella onedensis MR-1 to the environment stress.And the cytochrome C,nitrore-ductase NfsB and lactate utilization protein played key roles in the degradation and transformation of fluoxetine.This re-search enhances our understanding of the mechanism of fluoxetine degradation at the transcriptome and suggested a poten-tial application of Shewanella oneidensis MR-1 in environmental remediation.
关键词
Shewanella/oneidensis/MR-1/氟西汀/转录组/差异表达基因/功能基因Key words
Shewanella oneidensis MR-1/fluoxetine/transcriptome/differentially expressed genes/functional genes引用本文复制引用
基金项目
中央级公益性科研院所基本科研业务费专项生态环境部南京环境科学研究所项目(GYZX230304)
国家自然科学基金(41401360)
出版年
2024
国家科技期刊平台
NSTL
万方数据