Transcriptomic and Functional Genes Analyses of Shewanella oneidensis MR-1 upon Degradation of Fluoxetine
This paper studied the degradation performance of fluoxetine by Shewanella oneidensis MR-1 under anaerobic conditions and analyzed the genetic molecular metabolic mechanism from transcriptome.The removal rate of fluoxetine was 93.12%within 12 h,and the degradation rate was 0.94 mg·L-1·h-1.The transcriptome sequencing of Shewanella onei-densis MR-1 was performed using the Illumina high-throughput sequencing platform to understand the degradation mecha-nism of fluoxetine.Profiling and functional analysis of differentially expressed genes for tolerance and fluoxetine degradation using Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)revealed that envelope stress re-sponse membrane protein,ABC transporter,phage shock protein PspA and oxidative stress defense protein played important roles in the tolerance of Shewanella onedensis MR-1 to the environment stress.And the cytochrome C,nitrore-ductase NfsB and lactate utilization protein played key roles in the degradation and transformation of fluoxetine.This re-search enhances our understanding of the mechanism of fluoxetine degradation at the transcriptome and suggested a poten-tial application of Shewanella oneidensis MR-1 in environmental remediation.
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