灰葡萄孢霉菌产脱落酸工艺优化
Optimization of the Process of Producing Shedding Acid from Gray Grape Spore Mold
李欣泉1
作者信息
- 1. 江西新瑞丰生化股份有限公司 江西,吉安 331300
- 折叠
摘要
灰葡萄孢霉菌是一种重要的病原真菌,其生物学特征对ABA合成具有重要影响.利用CRISPR/Cas9等基因降低ABA合成途径中关键酶,可提高ABA含量和纯度.笔者探讨了利用代谢工程技术调控灰葡萄孢霉菌的代谢网络,实现对其代谢通路的改造与优化,提升其合成代谢能力.通过用反应曲面方法(RSM)对基质组成进行优化,并通过调控温度、pH值、溶氧等参数,对ABA的生物合成进行优化.在此基础上,改进灰葡萄孢霉菌发酵工艺,使其在ABA合成中的含量及纯度得到显著提高,同时也可降低生产成本,拓展灰葡萄孢霉菌的使用范围.
Abstract
Grey grape spore mold is an important pathogenic fungus,and its biological characteristics have an important impact on ABA synthesis.Using genes such as CRISPR/Cas9 to reduce key enzymes in the ABA synthesis pathway can improve the content and purity of ABA.The author discusses the use of metabolic engineering technology to regulate the metabolic network of gray grape spore mold,realize the transformation and optimization of its metabolic pathway,and improve its anabolic ability.The matrix composition is optimized by the reaction surface method(RSM),and the biosynthesis of ABA is optimized by regulating temperature,pH,dissolved oxygen and other parameters.On this basis,through the optimization of the fermentation process of gray grape spore mold,its content and purity in ABA synthesis can be significantly improved.At the same time,it can also reduce production costs and expand the use range of gray grape spore mold.
关键词
灰葡萄孢霉菌/产脱落/发酵工艺优化Key words
gray grape spore mold/production shedding/fermentation process optimization引用本文复制引用
基金项目
江西省科技厅重点研发项目(20181BBF60029)
出版年
2024
国家科技期刊平台
NSTL
万方数据