[Objective]Seeds are the basis of plant reproduction.To explore the morphological characteristics and germination rules of Polygonatum humile at different harvesting periods,in order to reveal the morphological characteristics,variation and suitable harvesting period of bamboo seeds,and provide scientific basis for cultivating high-quality seedlings of Polygonatum humile.[Methods]The wild Polygonatum humile in Dongtuanshan,Jilin City were taken as the research object,and the size characteristics,such as long diameter,wide diameter,longitudinal diameter,morphological index,size index and 1000-grain weight,were accurately measured at different development stages.The allometry relationship of seed morphological characteristics was analyzed by standard spindle estimation method.The seed germination was studied after 30 days of low temperature sand storage and germination on petri dish paper.[Results]The variation of seed size traits at different development stages was a continuous distribution of quantitative traits.The variation coefficient at development stage was weight>longitudinal diameter>wide diameter>long diameter,and the variation coefficient at maturity stage was concentrated in weight>long diameter>longitudinal diameter>wide diameter.Sand storage at 4℃for 30 days significantly increased germination rate and germination potential.[Conclusion]The seeds of Polygonatum humile tend to be elliptical,and the larger and fuller shapes tend to be"round balls"in three dimensions.The seed weight variation is the largest,which can be used for classification.The harvest at the end of September is more suitable,and the germination is faster and more orderly,and the germination rate can reach 87.78% .The results can guide the industrial practice and promote the development of sexual reproduction of Polygonatum humile.
关键词
采收期/种子性状/变异系数/异速生长/发芽率/低温沙藏
Key words
harvesting period/seed character/coefficient of variation/allometry/germination rate/low temperature sand deposit