[Objective]In order to further explore the application of microorganisms in agriculture,this study explored the application effect of strain H6-1 in agriculture from two aspects of biocontrol and growth promotion.[Methods]In this study,a strain of bacteria,number H6-1,was provided.The strain was identified as Bacillus subtilis by 16S rRNA identification.The strain was tested against a variety of pathogenic fungi by plate face-off,multi-crop growth promotion experiment,and physiological and biochemical analysis were carried out.[Results]The strain could inhibit the growth of various pathogenic fungi,and the average inhibition rate was more than 70% .It can promote the growth of eucalyptus seedling and fast vegetable.It has the activities of protease,cellulase,catalase and ferric carrier,and can dissolve insoluble phosphorus.The catalase activity reached 333 μg·mL-1·min-1,and the soluble content of inorganic phosphorus was 51.7 mg·L-1.[Conclusion]Bacillus subtilis in this study has both biocontrol and growth promotion effects,and has high efficiency,broad spectrum antibacterial activity and diversified biological activity,which can promote crop growth and development,and has potential application prospects in the field of agricultural microorganisms.
关键词
枯草芽孢杆菌/抑菌率/生物防治增效菌/生防/促生
Key words
Bacillus subtilis/bacteriostatic rate/bio-control and bio-fertilizing bacteria/biocontrol/growth
维普
万方数据