Aconitase(ACO)is a key enzyme involved in reversible isomerization of citrate in tricarboxylic acid cycle.There are three isoforms of this enzyme(ACO1,ACO2,ACO3),among which ACO3 plays the crucial role.Other studies have found that the aconitase gene At ACO3 also has an important biological function to improve the model plant Arabidopsis thaliana in response to abiotic stress.This paper focuses on the role of At ACO3 in improving cold resistance,drought resistance and metal stress resistance,and provides references for further study of ACO3 and further development of plant stress resistance functional genes.