Study on cutting of juvenile stem pieces of Cinnamomum bodinierivar.citralifera tissue culture seedlings
Using semi-lignified annual branches from juvenile stem pieces of Cinnamomum bodinieri var.citralifera as mate-rials,the effects of IBA,cutting medium and cutting time on rooting of C.bodinieri var.citralifera were studied with stem pieces cutting technique.The results show that the stem pieces'root rate was 89.58%from juvenile stem pieces of C.bodi-nieri var.citralifera tissue culture seedlings,and the rooting rate,root number and root length were significantly higher than those from cutting seedlings.IBA could significantly promote the juvenile stem pieces'rooting of C.bodinieri var.citralifera,the optimum concentration of IBA was 3000 mg/L,and the rooting rate reached 94.79%.The juvenile stem pieces'root rate of C.bodinieri var.citralifera from control group also reached 67.71%,the rooting rate was higher than those from cut-ting seedlings(54.17%),indicating that C.bodinieri var.citralifera tissue culture seedlings were induced to restore rooting ability.The mixture medium of vermiculite and pine bark had significant effect on the juvenile stem pieces'rooting of C.bo-dinieri var.citralifera,the optimum mixture medium of vermiculite and pine bark was in the ratio of 2∶1 and 1∶1,and the ju-venile stem pieces'root rate was higher,reaching 94.79%and 91.67%respectively.The cutting time also had significant effect on the juvenile stem pieces'rooting of C.bodinieri var.citralifer.The juvenile stem pieces'root rate reached 85%at the end of May and July,and the juvenile stem pieces'root rate was slightly low at the end of September(72.92%).
国家科技期刊平台
NSTL
万方数据
