首页|饲料中添加复方中草药对凡纳滨对虾生长、消化酶和免疫相关酶活性的影响

饲料中添加复方中草药对凡纳滨对虾生长、消化酶和免疫相关酶活性的影响

扫码查看
试验选取以黄芪(Astragalus membranaceus)、板蓝根(Baphicacanthus cusia)等为主要成分的中草药饲料添加剂配制5组饲料[添加量分别为0(对照组)、0.1%、0.2%、0.4%和0.8%],连续投喂凡纳滨对虾(Litopenaeus vannamei)[初始体质量为(4.14 ±0.06)g] 56 d,每组3个重复,研究复方中草药对凡纳滨对虾生长、消化酶活性和免疫相关酶活性的影响.结果显示,试验组对虾的成活率均高于对照组,其中0.2%组差异显著(P<0.05);0.2%组和0.8%组的相对增重率显著高于对照组(P<0.05).试验组肝胰脏和肠道的消化酶活性均高于对照组,其中0.2%组的肝蛋白酶和肝淀粉酶显著增高(P<0.05),0.1%组和0.4%组的肠蛋白酶和肠淀粉酶显著增高(P<0.05).0.2%组的碱性磷酸酶(AKP)活性显著高于其他组(P<0.05);试验组的超氧化物歧化酶(SOD)活性均高于对照组;随着中草药添加量的增加,过氧化物酶(POD)指标呈现先下降后增高的趋势,0.4%组显著降低(P<0.05).结果表明,饲料中添加复方中草药能促进凡纳滨对虾生长、消化酶和免疫相关酶活性,适宜添加量为0.2%.
Effects of Chinese herbal compound in feed on growth, digestive enzyme and immune-related enzyme activities of Litopennaus vannamei
To investigate the effects of Chinese herbal compound on the growth, digestive enzyme activities and immune-related enzyme activities of litopenaeus vannamei, five groups with 3 replicates per treatment of feed containing Chinese herbal feed additives (mainly Astragalus membranaceiu and Baphicacanthus cusia) at 0, 0.1% , 0. 2% , 0. 4% and 0. 8% , respectively, were fed to L vannamei [ initial weight (4. 14 ±0. 06) g] for 56 d. Results reveal that the survival of L vannamei in test groups is higher than that in the control , significantly higher in 0. 2% group ( P < 0. 05 ). The relative weight gain in 0. 2% and 0. 8% groups is significantly higher thanthat in the control (P < 0. 05). The digestive enzyme activities in the liver and intestine in the test groups are significantly higher than those in the control, significantly higher in 0. 2% group (P<0. 05). The intestinal cathepsin and amylase in the 0. 1% and 0. 4% groups are higher than those in the control ( P < 0. 05 ). The AKP in 0. 2% group is significantly higher than that in the other groups ( P < 0. 05). The SOD in the test groups is higher than that in the control. The POD decreases and then increases with increasing a-mount of Chinese herbs, especially that 0.4% group decreases significantly (P < 0. 05). In conclusion, Chinese herbal compound in feed ( optimum supplemental level: 0. 2% ) can improve the growth, digestive enzyme activities and immune-related enzyme activities of L. Vannamei.

Chinese herbsLitopennaus vannameigrowthdigestiveimmunity

文国樑、林黑着、李卓佳、陆鑫、袁丰华

展开 >

中国水产科学研究院南海水产研究所,广东省渔业生态环境重点实验室,农业部南海渔业资源开发利用重点实验室,广东广州510300

大连水产学院生命与科学技术学院,辽宁大连116023

广东海洋大学水产学院,广东湛江524088

展开 >

中草药 凡纳滨对虾 生长 消化 免疫

现代农业(虾)产业技术体系建设专项资金公益性行业(农业)专项国家科技支撑计划广东省科技计划项目广东省科技计划项目广东省鱼病防治专项

CARS-472011030342011BAD13B102006A203010042008A020100014

2012

南方水产科学
中国水产科学研究院南海水产研究所

南方水产科学

CSTPCDCSCD北大核心
影响因子:1.591
ISSN:2095-0780
年,卷(期):2012.8(2)
  • 28
  • 20