首页|基于形态学和宏基因组学的养殖蟹塘浮游植物群落分析

基于形态学和宏基因组学的养殖蟹塘浮游植物群落分析

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为全面准确评估中华绒螯蟹(Eriocheirsinensis)养殖池塘中后期水体浮游植物群落结构特征及其与环境因子的关系,于2022年6-10月逐月监测水质指标,同时采用基于形态学和宏基因组学的人工镜检和高通量测序两种方法分析浮游植物的群落结构特征,探讨浮游植物优势种与环境因子的关系.结果显示,人工镜检鉴定的浮游植物共4门65种,而高通量测序数据分析所得浮游植物共4门1463种,其中人工镜检鉴定的优势种为蓝藻门的水华微囊藻(Mi-crocystis flos-aquae)、细小隐球藻(Aphanocapsa elachista)以及硅藻门的中肋骨条藻(Skeletonema costatum),而高通量测序数据分析所得优势种为蓝藻门的水华束丝藻(Aphanizomenon flos-aquae)、铜绿微囊藻(M.aeruginosa).人工镜检方法计算所得多样性指数普遍低于高通量测序结果.两种方法共同分析得出溶解氧(DO)、叶绿素a(Chl-a)、总氮(TN)和氨氮(NH3-N)是影响浮游植物群落结构的关键环境因子.建议采用人工镜检与高通量测序法相结合的方式分析养殖期间的浮游植物群落结构,以便更全面、准确地评估养殖生态系统中的浮游植物群落结构特征及其与环境因子的关系.
Analysis of phytoplankton community in aquaculture crab pond using morphological and metagenomics methods
In order to fully and accurately evaluate the characteristics of phytoplankton community structure and its relation-ship with environmental factors in the middle and late periods of Chinese mitten crab(Eriocheir sinensis)pond culture,we had monitored the water quality indexes and phytoplankton community of aquaculture crab ponds from June to October 2022,and identified the phytoplankton community by artificial microscopy and high-throughput sequencing based on morphological and metagenomics methods.Then we discussed the relationship between dominant phytoplankton species and environmental factors.The results show that 65 species in four phyla were identified by artificial microscopy,and 1463 species by metagenomic data analysis,in which the dominant species identified by artificial microscopy were Microcystis flos-aquae,Aphanocapsa elachista and Skeletonema costatum,and the dominant species identified by metagenomic data analysis were Aphanizomenonflos-aquae and M.aeruginosa.The diversity index calculated by manual microscopy was generally lower than that of metage-nomic results.Dissolved oxygen,chlorophyll a,total nitrogen and ammonia nitrogen were the key environmental factors affect-ing phytoplankton community structure.It is suggested to simutaneously use artificial microscopy and high-throughput sequenc-ing methods to analyze phytoplankton community structure in aquacultural water,so as to evaluate the characteristics of phyto-plankton community structure and its relationship with environmental factors in aquaculture ecosystems more comprehen-sively and accurately.

Aquaculture crab pondPhytoplanktonCommunities characteristicsManual microscopyHigh-throughput se-quencingDiversity indexEnvironmental factor

张旭、周丽、蔡敏、崔娜欣、邹国燕、赵志勇、袁泉、黄伟伟、张亚雷

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上海市农业科学院生态环境保护研究所/上海低碳农业工程技术研究中心,上海 201403

同济大学环境科学与工程学院/污染控制与资源化研究国家重点实验室,上海 200092

上海市农业科学院农产品质量标准与检测技术研究所,上海 201403

养殖蟹塘 浮游植物 群落特征 人工镜检 高通量测序 多样性指数 环境因子

长江生态环境保护修复联合研究项目(第二期)国家重点研发计划上海市农科院卓越团队建设计划

2022-LHYJ-0203042021YFC3201503沪农科卓[2022]023

2024

10.12131/20230141

南方水产科学
中国水产科学研究院南海水产研究所

南方水产科学

CSTPCD北大核心
影响因子：1.591
ISSN：2095-0780
年,卷(期)：2024.20(1)
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