Effect of carbonate alkalinity on embryonic development and larval vita-lity of Pramisgurnus dabryanus
To investigate the adaptation of the embryos and larvae of Paramisgurnus dabryanus to carbonate alkalinity,we cul-tured the embryos which had developed to the blastocyst stage in water at carbonate alkalinities of 0,10,20,30 and 40 mmol·L-1,so as to investigate the hatching period,hatching rate,abnormality rate,survival rate,specific growth rate(SGR),yolk sac ab-sorption and survival activity index(SAI).The results show that:1)The embryo hatching rate was highest at carbonate alkalinity of 10 mmol·L-1 without significant difference compared with that of the control group(P>0.05).The hatching period was signi-ficantly shorter than that of the control group at carbonate alkalinities of 10 and 20 mmol·L-1(P<0.05).2)The abnormality rate of the newly hatched larvae ascended gradually with the increase of carbonate alkalinity,while the survival rate of larvae of 3 dah(Days after hatching)gradually decreased.3)The total length of newly hatched larvae decreased and then increased,while the total length of larvae of 3 dah increased and then decreased with the increase of carbonate alkalinity.The SGR and yolk sac ab-sorption rate showed an increasing and then decreasing tendency.4)The larval SAI first increased and then decreased with the increase of carbonate alkalinity and reached the maximum in 10 mmol·L-1 group.It is indicated that the optimal carbonate alka-linity for the embryonic development and larval growth of P.dableanus is 0-10 mmol·L-1.