Effects of pre-transport density and temperature domestication on simulated transport of juvenile Ictalurus punctatus
To explore the effects of different pre-transport acclimation methods on water quality,biochemical parameters and tissue structure of juvenile Ictalurus punctatus during transportation,and to provide references for transportation of juvenile I.punctatus,we conducted pre-transport intensive acclimation and temperature acclimation.The pre-transport intensive accli-mation involved fish-to-water ratios of 1∶2,1∶3 and 1∶4,and the temperature acclimation was at 14,19 and 24 ℃.The simu-lation transport lasted for 18 h,and we investigated the changes in water quality(Ammonia nitrogen,pH),biochemical parame-ters[Glucose(Glu)、cortisol(Cor),lactate dehydrogenase(LDH),catalase(CAT),superoxide dismutase(SOD),malondialde-hyde(MDA)]as well as organizational structure(Skin and intestine)at different time(0th,2nd,6th,18th hour)and 24-hour reco-very after transport.The results show that pre-transport intensive acclimation with a fish-to-water ratio of 1:4 and a tempera-ture of 19 ℃ had lower ammonia nitrogen levels than the other treatments(P＜0.05).Glu,Cor,LDH,CAT,SOD and MDA levels were higher than the other groups,and there was minimal damage to the skin and intestinal structures.In conclusion,pre-trans-port intensive acclimation with a fish-to-water ratio of 1∶4 and a temperature of 19 ℃ can improve anti-stress capacity effec-tively during transportation of juvenile I.punctatus.
Ictalurus punctatusDomesticationDensityTemperatureSimulated transport
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