首页|三丁酸甘油酯提高凡纳滨对虾鳃组织抗周期性高温胁迫能力的研究

三丁酸甘油酯提高凡纳滨对虾鳃组织抗周期性高温胁迫能力的研究

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在水产养殖中,周期性高温(Periodic high temperature,PHT)对虾类的生物学功能会产生显著影响.鳃作为虾类的呼吸和代谢器官,在维持体内渗透平衡方面具有关键作用,但其功能易受高温等环境变化的干扰.为了探究有效的对虾PHT应对策略,研究了三丁酸甘油酯(Tributyrin,TB)对PHT[(34±1)℃,7 d]胁迫下凡纳滨对虾(Litopenaeus van-namei)鳃组织形态和生理功能的影响.结果表明,PHT胁迫导致了显著的鳃组织损伤,诱导了氧化应激反应,进而造成内质网应激、细胞凋亡以及渗透调节功能障碍.不同饲喂策略下(胁迫前饲喂三丁酸甘油酯7 d;每天饲喂三丁酸甘油酯;胁迫后饲喂三丁酸甘油酯7d),鳃组织损伤程度均显著减轻,同时氧化应激水平降低.此外,三丁酸甘油酯还正向调节了 PHT诱导的氧化应激、内质网应激、细胞凋亡以及渗透调节基因的相对表达水平.尤其是每天饲喂三丁酸甘油酯能够有效保持鳃组织形态完整和生理功能稳定.
Study on tributyrin enhancing anti periodic high temperature stress ability of gill tissue in Litopenaeus vannamei
Periodic high temperature(PHT)has a significant effect on biological function of shrimps in aquaculture.Gill,as the respiratory and metabolic organ of shrimp,plays a key role in maintaining the osmotic balance in fish body.However,its func-tion is easily disturbed by environmental changes such as high temperature.In order to investigate effective strategies against PHT in shrimp,we investigated the effects of tributyrin(TB)on the morphology and physiological function of gill tissue of Litopenaeus vannamei under PHT[(34±1)℃,7 d]stress.The results show that PHT stress caused significant gill tissue damage and induced oxidative stress,which in turn caused endoplasmic reticulum stress,apoptosis and osmotic regulation dysfunction.With different feeding strategies(Feeding tributyrin for 7 d before stress,feeding tributyrin every day including 7 stressed days,and feeding tributyrin for 7 d after stress),the degree of gill tissue damage decreased significantly,so did the level of oxidative stress.In addition,tributyrin positively regulated the relative expression levels of oxidative stress,endoplasmic reticulum stress,apoptosis and osmoregulation genes which were induced by PHT.In particular,daily feeding tributyrin was effective in main-taining the morphological integrity of gill tissues and stable physiological functions.

Litopenaeus vannameiTributyrinPeriodic high temperatureGillHistologic changesPhysiological function

朱轩仪、郑晓婷、邢逸夫、黄建华、董宏标、张家松

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上海海洋大学水产与生命学院,上海 201306

中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室,广东广州 510300

中国水产科学研究院南海水产研究所深圳试验基地,广东深圳 518121

凡纳滨对虾 三丁酸甘油酯 周期性高温 鳃 组织学变化 生理功能

宁波市重点研发计划项目广东省海外大师项目(2021)中国-东盟海上合作基金项目中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助中国水产科学研究院基本科研业务费中国水产科学研究院基本科研业务费广东省驻镇帮镇扶村科技特派员项目

2023Z1132021SD192019XT04032021XT06KTP20210259

2024

10.12131/20230246

南方水产科学
中国水产科学研究院南海水产研究所

南方水产科学

CSTPCD北大核心
影响因子：1.591
ISSN：2095-0780
年,卷(期)：2024.20(3)
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