Transcriptome comparative analysis of liver tissues of three plateau Schizothoracinae fish species
The extreme environmental features of low temperature and low oxygen on the Qinghai-Tibet Plateau provide a na-tural laboratory for the evolution of biological adaptability.The subfamily Schizothoracinae are widely distributed in the water bodies surrounding the Qinghai-Tibet Plateau,and has adapted to the unique highland environment through long-term evolu-tionary process.In this study,we focused on two high-altitude(＞3 000 m)Schizothoracinae fish species(Schizothorax macropo-gon and S.waltoni)and one low-altitude(＜1 000 m)ancestral Schizothoracinae fish species(S.prenanti)to conduct a transcrip-tome sequencing comparative analysis regarding the liver tissues,which is the main energy metabolism organ in fish species.This analysis reveals the key signaling pathways involved in the adaptation of highland Schizothoracinae to high-altitude environ-ment.Comparing the liver transcriptomes of high-altitude with those of low-altitude Schizothoracinae,we identified 106 genes that shared differential changes in expression between the two high-altitude species.Among these genes,66 were upregulated and 40 were downregulated.GO and KEGG enrichment analysis reveals that the most important pathways for high-altitude adap-tation in Schizothoracinae include oxidation-reduction processes,pathways related to blood development,amino acid metabo-lism and steroid biosynthesis.Among them,genes related to oxidation-reduction and blood development(faxdc2,cpox,cyp51,and NADH-cytochrome b5 reductase 2)with high expression levels might play a crucial role in high-altitude adaptation.The study provides new insights into the molecular mechanisms underlying the adaptation of fish to high-altitude environment.
万方数据
维普
