首页|季节变化对珠江口棘头梅童鱼肠道和水体中细菌群落结构的影响

季节变化对珠江口棘头梅童鱼肠道和水体中细菌群落结构的影响

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棘头梅童鱼(Collichthys lucidus)是珠江口常见的优势种和经济鱼类物种.为揭示棘头梅童鱼的生物生态学特征,利用16SrRNA基因的高通量测序技术,探究了季节变化对珠江口棘头梅童鱼肠道和水体中细菌群落结构的影响.结果表明,鱼类肠道细菌以变形菌门居多(78.89％),其次为厚壁菌门(6.70％)和拟杆菌门(5.45％);水体细菌以变形菌门(40.32％)和拟杆菌门(37.97％)为主.棘头梅童鱼肠道中的优势菌属存在明显季节变化,春、夏季鱼类肠道中优势细菌为嗜冷杆菌(Psychrobacter),秋季为发光杆菌(Photobacterium),而冬季为假单胞菌(Pseudomonas)、Sva0996_marine_group、弧菌(Vibrio)和甲基杆菌(Methylobacterium).冬季棘头梅童鱼肠道菌群的α多样性指数最高,秋季最低.春季和夏季鱼类肠道细菌组成无显著性差异(p＞0.05),而其余季节间存在显著性差异(p＜0.05).水体细菌群落组成的季节变化与鱼类肠道菌群变化相似.水环境中黄杆菌(Tenacibaculum)、嗜冷杆菌(Psychrobacter)、Candidatus_Actinomarina、Salinimonas和OM60NOR5_clade共5个菌属与鱼类肠道细菌组成之间存在显著相关性(r＞0.4,p＜0.01).细菌共现网络分析显示冬季鱼类肠道和水环境的共有菌群比例最低,而夏季最高.研究表明,季节变化对珠江口棘头梅童鱼的肠道菌群和水体细菌群落组成均存在影响.
Seasonal effects on bacterial community between intestine of Collichthys lucidus and water environment from Pearl River Estuary
Collichthys lucidus is a common dominant and economically important fish species in the Pearl River Estuary.To reveal the ecological characteristics of C.lucidus,we applied high-throughput sequencing technology of 16S rRNA gene to investi-gate the effects of seasonal changes on the bacterial community structure in the intestines and water body of the Pearl River Estuary.The bacterial community in the fish intestines was predominantly composed of members of Proteobacteria(78.89％),followed by Firmicutes(6.70％)and Bacteroidota(5.45％),while Proteobacteria(40.32％)and Bacteroidota(37.97％)domi-nated the surrounding water.Besides,we observed the seasonal changes in the dominant bacterial genera in the intestine of C.lucidus.The relative abundance of Psychrobacter was significantly higher in spring and summer,whereas Photobacterium predominated in autumn.However,Pseudomonas,Sva0996 marine group,Vibrio and Methylobacterium were more abundant in winter.The α-diversity index values of the intestinal bacteria of C.lucidus were highest in winter but lowest in autumn.PCoA and ANOSIM analyses reveal no significant differences in the intestinal bacterial composition between spring and summer(p＞0.05),whereas significant differences were observed in the other seasons(p＜0.05).The seasonal changes in the bacterial com-position of surrounding water were similar to those in the fish intestinal bacteria.Mantel test analysis finds out that five bacterial genera(Tenacibaculum,Psychrobacter,Candidatus_Actinomarina,Salinimonas,OM60NOR5_clade)in water environment showed a significantly positive correlation with the intestinal bacteria of C.lucidus(r＞0.4,p＜0.01).Bacterial co-occurrence net-work analysis shows that the proportion of shared bacterial communities between the fish intestines and the water environment was lowest in winter but highest in summer.The results indicate that seasonal changes can impact both the intestinal bacterial communities of C.lucidus from the Pearl River Estuary and bacterial communities in water body.

Collichthys lucidusIntestinal bacterial communityCo-occurrence network16S rRNA genePearl River Estuary

郑秋实、刘永、李纯厚、吴鹏、肖雅元、林琳、刘玉、邹剑

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浙江海洋大学水产学院,浙江舟山 316022

中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室/广东珠江口生态系统野外科学观测研究站/广东省渔业生态环境重点实验室,广东广州 510300

棘头梅童鱼 肠道菌群 共现网络 16SrRNA基因 珠江口

广州市科技计划项目农业财政专项项目广东省基础与应用基础研究基金项目中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助珠海市社会发展领域科技计划项目

202102080509NHZX20242022A15150108962023TD162021SD042020SY012320004343

2024

10.12131/20240030

南方水产科学
中国水产科学研究院南海水产研究所

南方水产科学

CSTPCD北大核心
影响因子：1.591
ISSN：2095-0780
年,卷(期)：2024.20(4)