Effects of water temperature in transportation on mortality and physiolo-gical indicators of Sepiella japonica
Restocking and releasing is an important means to restore the resources of Sepiella japonica,and the water tempera-ture during the transportation process of restocking and releasing may affect its survival status.In order to optimize the trans-portation techniques for the adult enrichment and release of S.japonica,we conducted an experiment to simulate the transpor-tation environment in laboratory and performed transportation experiments of adult S.japonica with different water tempera-tures(14,17,20,23,26,29 ℃),and explored its mortality rate and liver physiological indicators.The results show that the water temperature in transportation affected the mortality rate significantly(p<0.05),and the mortality rates in 14 and 29 ℃ groups were higher,reaching 30.0%and 63.3%,respectively.Compared with 20 ℃ group,the glycogen content decreased signifi-cantly in 14 and 17 ℃ groups,but increased significantly in 29 ℃ group(p<0.05).The lactic acid content increased signifi-cantly in 23 and 29 ℃ groups(p<0.05).SOD decreased significantly in 17 ℃ group but increased significantly in 26 ℃ group(p<0.05).AKP decreased significantly at all water temperatures(p<0.05).With the change of water temperature,AST decreased initially and then increased,while ALT increased initially and then decreased.The mortality rate of squids was significantly posi-tively correlated with glycogen,lactic acid content and AST activity in liver(p<0.05).In conclusion,too low or too high water temperature is not suitable for squid transportation at a density of 5 ind·L-1,and it is recommended to control the water tempera-ture between 17 ℃ and 26 ℃.