南方水产科学2024,Vol.20Issue(4) :107-115.DOI:10.12131/20240089

运输水温对曼氏无针乌贼成体死亡率及生理指标的影响

Effects of water temperature in transportation on mortality and physiolo-gical indicators of Sepiella japonica

蒋永生 周珊珊 周永东 徐开达 镇小蔓 焦礼诗 张秋宏 渠遥
南方水产科学2024,Vol.20Issue(4) :107-115.DOI:10.12131/20240089

运输水温对曼氏无针乌贼成体死亡率及生理指标的影响

Effects of water temperature in transportation on mortality and physiolo-gical indicators of Sepiella japonica

蒋永生 1周珊珊 2周永东 2徐开达 2镇小蔓 1焦礼诗 1张秋宏 1渠遥1
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作者信息

  • 1. 浙江海洋大学海洋与渔业研究所,浙江舟山 316021
  • 2. 浙江海洋大学海洋与渔业研究所,浙江舟山 316021;浙江省海洋水产研究所/农业农村部重点渔场渔业资源科学观测实验站/浙江省海洋渔业资源可持续利用技术研究重点实验室,浙江舟山 316021
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摘要

增殖放流是曼氏无针乌贼(Sepiella japonica)渔业资源修复的重要手段,而水温可能会影响增殖放流运输过程中乌贼的生存状态.为优化曼氏无针乌贼成体增殖放流运输技术,通过实验室内模拟不同水温(14、17、20、23、26、29℃)下的运输过程,分析了不同运输温度下乌贼死亡率和肝脏生理指标的变化.结果显示,运输水温显著影响乌贼成体死亡率(p<0.05),14和29℃处理组的死亡率较高,分别为30.0%和63.3%.相较于20℃处理组,肝糖原含量在14和17℃下显著下降,在29℃下显著上升(p<0.05);乳酸含量在23和29℃下显著上升(p<0.05);超氧化物歧化酶(SOD)活性在17 ℃下显著下降,在26℃下显著上升(p<0.05);碱性磷酸酶(AKP)活性在各水温下显著下降(p<0.05);随水温变化,谷草转氨酶(AST)活性先下降后上升,而谷丙转氨酶(ALT)活性则先上升后下降.乌贼死亡率与肝脏糖原、乳酸含量和AST活性显著正相关(p<0.05).研究表明,在5只·L-1密度下水温过低或过高均不适宜乌贼运输,建议水温控制在17~26 ℃.

Abstract

Restocking and releasing is an important means to restore the resources of Sepiella japonica,and the water tempera-ture during the transportation process of restocking and releasing may affect its survival status.In order to optimize the trans-portation techniques for the adult enrichment and release of S.japonica,we conducted an experiment to simulate the transpor-tation environment in laboratory and performed transportation experiments of adult S.japonica with different water tempera-tures(14,17,20,23,26,29 ℃),and explored its mortality rate and liver physiological indicators.The results show that the water temperature in transportation affected the mortality rate significantly(p<0.05),and the mortality rates in 14 and 29 ℃ groups were higher,reaching 30.0%and 63.3%,respectively.Compared with 20 ℃ group,the glycogen content decreased signifi-cantly in 14 and 17 ℃ groups,but increased significantly in 29 ℃ group(p<0.05).The lactic acid content increased signifi-cantly in 23 and 29 ℃ groups(p<0.05).SOD decreased significantly in 17 ℃ group but increased significantly in 26 ℃ group(p<0.05).AKP decreased significantly at all water temperatures(p<0.05).With the change of water temperature,AST decreased initially and then increased,while ALT increased initially and then decreased.The mortality rate of squids was significantly posi-tively correlated with glycogen,lactic acid content and AST activity in liver(p<0.05).In conclusion,too low or too high water temperature is not suitable for squid transportation at a density of 5 ind·L-1,and it is recommended to control the water tempera-ture between 17 ℃ and 26 ℃.

关键词

曼氏无针乌贼/模拟运输/水温/死亡率/生理指标

Key words

Sepiella japonica/Simulated transportation/Water temperature/Mortality/Physiological indicators

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基金项目

国家重点研发计划"蓝色粮仓科技创新"重大专项(2020YFD09008004)

国家重点研发计划"蓝色粮仓科技创新"重大专项(2019YFD0901204)

国家重点研发计划项目(2023YFD2401900)

浙江省重点研发计划项目(2019C02056)

出版年

2024
南方水产科学
中国水产科学研究院南海水产研究所

南方水产科学

CSTPCDCSCD北大核心
影响因子:1.591
ISSN:2095-0780
参考文献量57
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