The Effect of FBXO22 expression on biological behavior of HPV positive cervical cancer cells
Aim To explore the effect of FBXO22 expression on the biological behavior of human papilloma virus(HPV)positive cervical cancer cells.Methods qRT-PCR and Western blotting were used to detect the differential expression of FBXO22 in H8 cells and cervical cancer cell lines.Hela and CaSki cells with FBXO22 knocked out and overexpressed were constructed in the experimental group,and the control group was transferred to empty vector.qRT-PCR and Western blotting were used to detect the ex-pression of FBXO22 after transfection.MTT assay,cell clone formation test,flow cytometry,scratch test and Transwell test were used to detect the effects of FBXO22 expression on cell proliferation,cell cycle,migration and invasion.Results Compared with H8 cells,FBXO22 was overexpressed in HPV positive cervical cancer cells(P＜0.05).Compared with the control group,the mRNA and protein of FBXO22,cell proliferation activity and cell clone formation rate of Hela and CaSki cells increased in the over-expression group,but decreased in the knock-down group(P＜0.05).In G2/M phase,the percentage of cells,the width of scratches and the number of transmembrane cells decreased in FBXO22 overexpression group,but increased in knock-down group(P＜0.05).Con-clusion FBXO22 is overexpressed in HPV positive cervical cancer cells.The expression of FBXO22 promotes the proliferation and migration of cervical cancer cells,and silencing FBXO22 may provide a potential target for cervical cancer treatment.
万方数据
维普
