摘要
目的 探讨miR-519b-3p调控IL-6/信号转导和转录激活因子3(STAT3)通路对宫颈癌细胞(SiHa)凋亡、迁移、侵袭的影响.方法 qRT-PCR检测miR-519b-3p在宫颈癌组织中的表达.SiHa细胞分为miR-NC组、miR-519b-3p组、miR-NC+RhIL-6组、miR-519b-3p+RhIL-6组.流式细胞术评估SiHa细胞凋 亡率,Transwell实验检测细胞迁移和侵袭情况.Western blotting 检测 B 细胞淋巴瘤-2 基因(Bcl-2)、E-钙黏蛋白(E-cadherin)、N-cadherin、Bcl 相关 x 蛋白(Bax)、STAT3 和 p-STAT3 蛋白水平.结果 与癌旁组织比较,宫颈癌组织miR-519b-3p表达下调(P＜0.05).SiHa细胞凋亡率、Bax和E-cadherin蛋白水平 miR-519b-3p 组高于 miR-NC 组,miR-NC+RhIL-6 组低于 miR-NC 组,miR-519b-3p+RhIL-6 组低于 miR-519b-3p 组(P＜0.05).SiHa 细胞迁移数、侵袭数、Bcl-2、N-cadherin、p-STAT3 蛋白水平 miR-519b-3p 组低于 miR-NC 组,miR-NC+RhIL-6 组高于 miR-NC 组,miR-519b-3p+RhIL-6 组高于 miR-519b-3p 组(P＜0.05).结论 miR-519b-3p 通过抑制 IL-6/STAT3 通路来抑制宫颈癌细胞迁移和侵袭,并增加细胞凋亡.
Abstract
Aim To investigate the effect of miR-519b-3p on apoptosis,migration,and invasion of cervical cancer cells(Si-Ha)through the regulation of IL-6/signal transduction and transcription activating factor 3(STAT3)pathway.Methods qRT-PCR was used to detect the expression of miR-519b-3p in cervical cancer tissue.SiHa cells were divided into miR-NC group,miR-519b-3p group,miR-NC+RhIL-6 group,and miR-519b-3p+RhIL-6 group.Flow cytometry was used to evaluate the apoptosis rate of SiHa cells,while Transwell assay was used to detect cell migration and invasion.Western blotting was used to detect the levels of B-cell lymphomatoma-2 gene(Bcl-2),E-cadherin,N-cadherin,Bel related x protein(Bax),STAT3,and p-STAT3 protein.Re-sults Compared with adjacent tissues,the expression of miR-519b-3p in cervical cancer tissue was downregulated(P＜0.05).The apoptosis rate of SiHa cells,Bax and E-cadherin protein levels were higher in the miR-519b-3p group than in the miR-NC group,lower in the miR-NC+RhIL-6 group than in the miR-NC group,and lower in the miR-519b-3p+RhIL-6 group than in the miR-519b-3p group(P＜0.05).The migration number,invasion number,Bcl-2,N-cadherin,p-STAT3 protein levels of SiHa cells were lower in the miR-519b-3p group than in the miR-NC group,higher in the miR-NC+RhIL-6 group than in the miR-NC group,and higher in the miR-519b-3p+RhIL-6 group than in the miR-519b-3p group(P＜0.05).Conclusion miR-519b-3p inhibits the migration and in-vasion of cervical cancer cells and increases cell apoptosis by inhibiting the IL-6/STAT3 pathway.
维普
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