The correlation between NLRP3 inflammasome,inflammatory response,and pyroptosis mark-ers in patients with chronic periodontitis and Porphyromonas gingivalis infection
Aim To study the correlation between NLRP3 inflammasome,inflammatory response,and pyroptosis markers in patients with chronic periodontitis and Porphyromonas gingivalis infection.Methods 300 patients with chronic periodontitis were selected and divided into a positive group and a negative group based on the infection status of Porphyromonas gingivalis.The expres-sion levels of NLRP3,apoptosis-associated speck-like protein(ASC),Caspase-1 mRNA and GSDMD-N protein in periodontal tissue,as well as interleukin-1β(IL-1β),IL-8,tumor necrosis factor-α(TNF-α)in gingival crevicular fluid between two groups were meas-ured.Correlation analysis was done by using Pearson test.Results Plaque index(PLI),gingival index(GI),attachment loss(AL),pocket depth(PD),periodontal tissue NLRP3,ASC,Caspase-1 mRNA,GSDMD-N protein,and gingival crevicular fluid IL-1β、IL-18,TNF-α levels in positive groups were higher than those in the negative group(P＜0.05).The expression levels of NL-RP3,ASC,and Caspase-1 mRNA were positively correlated to PLI,GI,AL,PD,GSDMD-N proteins,IL-1 β,IL-18,and TNF-αlevels(P＜0.05).Conclusion The infection of Porphyromonas gingivalis in patients with chronic periodontitis exacerbates the condition,inflammatory response,and necrosis,and its effect may be related with the activation of NLRP3 inflammasomes.
万方数据
维普
