摘要
目的 研究慢性牙周炎牙龈卟啉单胞菌感染患者NLRP3炎症小体与炎症反应及焦亡指标的相关性.方法 选择300例慢性牙周炎患者,按照牙龈卟啉单胞菌感染情况分为阳性组和阴性组.比较两组牙周健康指标、牙周组织NLRP3、凋亡相关斑点样蛋白(ASC)、Caspase-1 mRNA和GSDMD-N蛋白表达水平,以及龈沟液白细胞介素(IL)-1β、IL-18、肿瘤坏死因子-α(TNF-α)水平.采用Pearson检验进行相关性分析.结果 阳性组菌斑指数(PLI)、牙龈指数(GI)、附着丧失(AL)、牙周袋深度(PD)、牙周组织NLRP3、ASC、Caspase-1 mRNA、GSDMD-N蛋白,以及龈沟液IL-1β、IL-18、TNF-α水平均高于阴性组(P＜0.05).NLRP3、ASC、Caspase-1 mRNA 表达水平与 PLI、GI、AL、PD、GSDMD-N 蛋白、IL-1β、IL-18、TNF-α 水平均呈正相关(P＜0.05).结论 慢性牙周炎患者牙龈卟啉单胞菌感染加重病情、炎症反应及细胞焦亡,其作用可能与激活NLRP3炎症小体有关.
Abstract
Aim To study the correlation between NLRP3 inflammasome,inflammatory response,and pyroptosis markers in patients with chronic periodontitis and Porphyromonas gingivalis infection.Methods 300 patients with chronic periodontitis were selected and divided into a positive group and a negative group based on the infection status of Porphyromonas gingivalis.The expres-sion levels of NLRP3,apoptosis-associated speck-like protein(ASC),Caspase-1 mRNA and GSDMD-N protein in periodontal tissue,as well as interleukin-1β(IL-1β),IL-8,tumor necrosis factor-α(TNF-α)in gingival crevicular fluid between two groups were meas-ured.Correlation analysis was done by using Pearson test.Results Plaque index(PLI),gingival index(GI),attachment loss(AL),pocket depth(PD),periodontal tissue NLRP3,ASC,Caspase-1 mRNA,GSDMD-N protein,and gingival crevicular fluid IL-1β、IL-18,TNF-α levels in positive groups were higher than those in the negative group(P＜0.05).The expression levels of NL-RP3,ASC,and Caspase-1 mRNA were positively correlated to PLI,GI,AL,PD,GSDMD-N proteins,IL-1 β,IL-18,and TNF-αlevels(P＜0.05).Conclusion The infection of Porphyromonas gingivalis in patients with chronic periodontitis exacerbates the condition,inflammatory response,and necrosis,and its effect may be related with the activation of NLRP3 inflammasomes.
维普
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