Allicin improves HUVEC autophagic damage induced by PM2.5 exposure
Aim To investigate the mechanism of allicin on PM2.5-induced autophagy in human umbilical vein endothelial cells(HUVEC).Methods HUVEC were divided into control group,PM2.5 group(5 mg/L),allicin group(10 mg/L)and alli-cin combined group(10 mg/L allicin and5 mg/L PM2.5).The number of autophagosomes in each group was observed by transmis-sion electron microscopy.The needle count of LC3,p62 and the protein expressions of p62,p38,Beclin 1,LC3BⅡ/Ⅰand the phos-phorylation level of p38 were detected by cell immunofluorescence and Western blotting.Results Compared with the control group,the cell viability of PM2.5 group was significantly decreased,whereas the number of autophagosomes was increased and the protein expres-sions of Beclin 1,LC3BⅡ/Ⅰand p62 were increased(P<0.05).Compared with the PM2.5 group,the cell viability of allicin combined group was increased,the number of autophagosomes was decreased,and the protein levels of Beclin 1,LC3BⅡ/Ⅰand p62,as well as the phosphorylation level of p38 were decreased(P<0.05).Conclusion Allicin improves PM2.5-induced HUVEC injury,which may be related with the inhibition of phosphorylation of p38 protein and promotion of autophagy flow inhibited by PM2.5.
国家科技期刊平台
NSTL
万方数据
