Atorvastatin improves myocardial energy metabolism in adriamycin-induced dilated cardio-myopathy rats
Aim To investigate the effect of atorvastatin on myocardial energy metabolism in adriamycin dilated cardiomyop-athy(DCM)rats.Methods DCM rat model was induced by 2.5 mg/kg adriamycin.Wistar rats were divided into control group,DCM group,and DCM+atorvastatin group(atf-DCM group).Cardiac structure and function,volume and mass were compared at the 7th week in each group;HE staining was used to observe the pathomorphological changes of rat myocardial tissue;Western blotting was used to detect the protein expression levels of peroxisome proliferator-activated receptor α(PPARα),PPAR cos-timulatory factor-1α(PGC-1α),and adenine nucleotide transporter(ANT)in rat myocardial tissue.Results Compared with the control group,the heart mass of DCM group increased,and atf-DCM group reversed the above trend(P<0.01).The heart volume of DCM rats was larger than that of control group(P<0.01).Cardiac structure and function were better in atf-DCM group than in DCM group,but did not reach the level of control group,and there was no significant difference between the groups(P>0.05).HE stai-ning showed that some myofibrils in the left ventricle of DCM group were dissolved,the myocardial cell space was increased,the myo-cardial fibers were broken,and vacuoles were produced;in the atf-DCM group,the myocardial fiber breakage was relieved,the myo-cardial cell space was not significantly increased,and vacuolar degeneration was not obvious.Myocardial PPARα,PGC-1α and ANT protein levels were lower in the DCM group than in the control group and higher in the atf-DCM group than in the DCM group(P<0.01).Conclusion Atorvastatin can up-regulate myocardial PPARα,PGC-1α and ANT protein levels,improve myocardial en-ergy metabolism,and thus improve cardiac function in DCM rats.
万方数据
维普
