Effects of miR-7 on Hp-induced autophagy and EMT in gastric epithelial cells
Aim To investigate the effect of microRNA-7(miR-7)on Helicobacter pylori(Hp)-induced autophagy and epi-thelial mesenchymal transition(EMT)in gastric epithelial cells(GES-1).Methods The Hp Sydney strain(SS1)was co cul-tured with GES-1 cells in vitro to establish a GES-1 cell transformation model.The experiment was divided into blank control group(NC group),Hp group,miR-7 mimics group,and Hp+miR-7 mimics group.The transfection effect was verified by qRT-PCR.MTT assay,scratch assay and Transwell assay were used to detect the proliferation,migration and invasion of GES-1 cells in each group.Western blotting was used to detect the expression of autophagy related proteins(RM-9106 microtubule related protein 1 light chain 3-Ⅰ(LC3-Ⅰ),LC3-Ⅱ)and epithelial mesenchymal transformation(EMT)related proteins(E-cadherin,N-cadherin,Vim-entin)in GES-1 cells in each group.Results Compared with the NC group,the Hp group showed a decrease in the proliferation inhibition rate,p62 protein,and E-Cadherin protein levels of GES-1 transformed cells(P<0.05),while the migration rate,invasion number,number of autophagosomes,total area of autophagic vesicles/cytoplasm,ratio of LC3-Ⅱ/LC3-Ⅰ,N-Cadherin and Vimentin protein levels increased(P<0.05).The miR-7 mimics group also showed an increase in the proliferation inhibition rate,p62 pro-tein,and E-Cadherin protein levels of GES-1 cells(P<0.05),and the migration rate The number of invasions,the number of autoph-agosomes,the total area of autophagic vesicles/cytoplasm,the ratio of LC3-Ⅱ/LC3-Ⅰ,and the levels of N-Cadherin and Vimentin proteins all decreased(P<0.05);Compared with the miR-7 mimics group,the Hp+miR-7 mimics group showed a decrease in the in-hibition rate of transformed cell proliferation,p62 protein levels,and E-Cadherin protein levels(P<0.05),while the migration rate,invasion number,number of autophagosomes,total area of autophagic vesicles/cytoplasm,ratio of autophagy related protein LC3-Ⅱ/LC3-Ⅰ,and levels of N-Cadherin and Vimentin proteins all increased(P<0.05).Conclusion miR-7 down-regulates Hp-in-duced GES-1 autophagy,reduces cell invasion and migration,and inhibits EMT.
万方数据
维普
